草酸青霉C11对地黄生长的影响及其代谢产物分析

Effects of Penicillium oxalicum C11 on Rehmannia glutinosa growth and its metabolites analysis

分离对地黄(Rehmannia glutinosa)生长有害的菌株并解析其代谢产物对地黄生长的影响,可为破解地黄连作障碍问题提供理论支持。从地黄重茬土壤中分离霉菌并研究其发酵液对地黄生长的影响,然后通过不同极性梯度萃取、硅胶柱层析方法分离对地黄危害最严重的代谢产物,并通过高效液相色谱和液相色谱-质谱联用技术鉴定分离出的物质。结果:筛选出1株草酸青霉(Penicillium oxalicum)菌株C11,分离鉴定到其有害化感物质是环(精-亮)二肽(分子量为269.347,分子式为:C12H23N5O2)。结果表明草酸青霉C11抑制了地黄产量41.5%,其有毒代谢产物环(精-亮)二肽可能是造成地黄连作障碍的原因。

Aims The aim of this study was to isolate the harmful strain and analyze the effects of its metabolites on Rehmannia glutinosa,which provided theoretical support for solving R.glutinosa continuous cropping obstacles.Methods The fungi were isolated from continuous cropping soil.The fungal fermentation liquid metabolites were separated using different polarity gradient extraction and silica gel column chromatography.Metabolites were determined by high performance liquid chromatography and liquid chromatography-mass spectrometry.Important findings A strain of Penicillium oxalicum C11 was isolated from continuous cropping soil.The toxic metabolite of P.oxalicum C11 was identified as cyclo(L-Arg-L-Leu)dipeptide(molecular weight:269.347,molecular formula:C12H23N5O2).Results indicated P.oxalicum C11 inhibited R.glutinosa yield by 41.5%,suggesting that the toxic metabolite L-Arg-L-Leu could be vital for R.glutinosa continuous cropping obstacles.