不同样地白木香结香前后根际土壤细菌群落结构差异与土壤理化性质相关性研究

Differences in Inter-root Soil Bacterial Community Structure and Soil Physicochemical Properties in Different Locations of Aquilaria sinensis

为阐明白木香结香前后根际细菌群落差异,并探讨与土壤理化性质的相关性。本研究采用高通量测序技术和生物信息学相关手段,对海南省3个种植地(海口市文山村、临高东光农场、乐东抱伦农场)白木香结香前后根际土壤细菌群落组成和多特征进行分析,并结合冗余分析对根际土壤理化性质与细菌群落进行相关性分析。结果表明:3个样地间共获得7944402条序列,隶属于51个门,151个纲,380个目,637个科,1296个属,2649个种;样地内结香前后细菌群落和多样性差异性不大,样地间存在显著差异。3个样地根际土壤细菌丰富度和多样性上均呈现为:LDJX>LDWJ>LGWJ>LGJX>WSWJ>WSJX。在群落结构上,乐东样地与文山和临高样地群落结构存在显著差异。在门水平上,乐东样地的优势细菌门为浮霉菌门(Planctomycetota)、绿弯菌门(Chloroflexi)、疣微菌门(Verrucomicrobiota);文山和临高样地的菌门为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、变形菌门(Proteobacteria)。在属水平上,文山和临高的优势属为拟杆菌属(Bacteroides)、uncultured_bacterium_f Muribaculaceae、粪杆菌属(Faecalibacterium);乐东优势属为未确定属(g_uncultured_f__Gemmataceae)、Candidatus_Udaeobacter和拟杆菌属(Bacteroides)。对RDA分析发现,不同样地间白木香根际土壤细菌群落受土壤理化因子的影响具有一定差异。全磷与细菌种群差异和多样性呈显著负相关,pH与细菌种群差异和多样性呈显著正相关。该研究结果对白木香根际微生物互作关系研究具有参考价值,对海南沉香产业发展具有指导意义。

To elucidate the differences in inter-root bacterial communities of Aquilaria sinensis,and to explore the cor-relation with soil physicochemical properties,we used the high-throughput sequencing technology and bioinformat-ics-related tools to analyze the composition and multi-characteristics of the inter-root soil bacterial community of A.sinensis.In three cultivation sites in Hainan province(Wenshan village,Haikou city,Dongguang farm,Lingao,and Baolun farm,Ledong),and combined with redundancy analysis to correlate the inter-root soil physicochemical proper-ties with the bacterial community.The results showed that a total of 7944402 sequences belonging to 51 phyla,151 orders,380 families,637 families,1296 genera and 2649 species were obtained among the sites;the variability of bac-terial community and diversity of A.sinensis in the sample sites was not significant,and there were significant differ-ences among the sample sites.The richness and diversity of inter-root soil bacteria in the sites were as follows:LDJX>LDWJ>LGWJ>LGJX>WSWJ>WSJX.In terms of community structure,the Ledong sample site differed sig-nificantly from the Wenshan and Lingao sample sites in terms of community structure.at the phylum level,the dominant bacterial phyla in Ledong were Planctomycetota,Chloroflexi,and Verrucomicrobiota;the dominant populations in Wenshan and Lingao were Firmicutes,Bacteroidota,and Proteobacteria.At the genus level,the dominant genera in Wenshan and Lingao were Bacteroides,uncultured_bacterium_f Muribaculaceae,and Faecalibacterium;the dominant genera in Ledong were the unidentified genus(g_uncultured_f__Gemmataceae)genus,Candidatus_Udaeobacter,and Bacteroides.The RDA analysis revealed that the inter-rhizosphere soil bacterial community of A.sinensis was affected by soil physicochemical factors with some differences among different sites.Total phosphorus was significantly and negatively correlated with bacterial population differences and diversity,and pH was significantly and positively corre-lated with bacterial population differences and diversity.The results are valuable for studying the interactions of in-ter-root microorganisms of A.sinensis,and have certain guiding significance for the development of Hainan incense industry.