宫颈癌组织中ANXA8、PGRMC1和miR-92a表达水平及其与临床病理特征的关系

Expression of ANXA8,PGRMC1,and miR-92a in Cervical Cancer and Their Relationship with Clinicopathological Features

目的 探究宫颈癌患者癌组织中膜联蛋白A8(annexin A8, ANXA8)、孕激素受体膜组分1(progesterone receptor membrane component 1,PGRMC1)以及微小核糖核酸-92a(micro RNA-92a,mi R-92a)的表达及其与临床病理特征的关系。方法 收集2020年1月至2022年3月宜宾市第二人民医院接治的69例宫颈癌患者的宫颈癌和癌旁正常组织69例,采用免疫组织化学法检测ANXA8和PGRMC1阳性率,Western blot检测ANXA8和PGRMC1蛋白表达,RT-PCR法检测ANXA8和PGRMC1 m RNA以及mi R-92a表达水平。此外,分析ANXA8、PGRMC1与mi R-92a表达的线性相关性及其与宫颈癌患者临床病理特征的关系。结果 宫颈癌组织中ANXA8和PGRMC1阳性率(76.81%、72.46%)高于癌旁正常组织(4.35%、7.25%),mi R-92a表达量高于癌旁正常组织(P <0.05)。FIGO分期越高、分化程度越低以及具有淋巴结转移和宫旁浸润,ANXA8和PGRMC1阳性率以及mi R-92a表达量越高(P <0.05)。结论 ANXA8、PGRMC1和mi R-92a在宫颈癌组织中高表达,与宫颈癌肿瘤分期、分化程度、转移和浸润等病理特征有关,可为宫颈癌患者的病理学评估提供可靠依据。

Objective To investigate the expression of ANXA8,PGRMC1,and miR-92a in cervical cancer tissues and their relationship with clinicopathological features.Methods A total of 69 cervical cancer patients treated at the Second People’sHospital of Yibin City from January 2020 to March 2022 were included in this study.Cervical cancer tissues and adjacent normal tissues from these patients were collected for analysis.Immunohistochemistry was used to detect the positivity of ANXA8 and PGRMC1.Western blot was performed to analyze the protein expression of ANXA8 and PGRMC1.RT-PCR was used to measure the mRNA levels of ANXA8 and PGRMC1,as well as the expression level of miR-92a.The linear correlation among ANXA8,PGRMC1,and miR-92a expression levels,as well as their relationships with the clinical pathological features of cervical cancer patients,were analyzed.Results The positive rates of ANXA8 and PGRMC1 in cervical cancer tissues(76.81%,72.46%)were significantly higher than adjacent tissues(4.35%and 7.25%),and the expression level of miR-92a was significantly higher than adjacent tissues(P<0.05).With higher FIGO stage,lower differentiation degree,lymph node metastasis,and parametrial infiltration,the positivity rates of ANXA8 and PGRMC1 as well as the expression level of miR-92a were higher(P<0.05).Conclusion ANXA8,PGRMC1,and miR-92a are highly expressed in cervical cancer tissues,and are related to the pathological characteristics such as tumor staging,differentiation degree,metastasis,and invasion.They can provide a reliable basis for the pathological evaluation of cervical cancer patients.