主动脉夹层患者主动脉壁中血清淀粉样P物质的表达变化及其潜在临床意义

Expression pattern of serum amyloid P component in aorta of patients with aortic dissection and its potential clinical relevance

目的探讨血清淀粉样P物质(SAP)在主动脉夹层患者主动脉壁组织中的表达变化及其潜在的临床意义。方法收集2020年1月至2021年12月在华中科技大学同济医学院附属同济医院心脏大血管外科明确诊断为Stanford A型主动脉夹层(TAAD)患者主动脉壁标本60例(夹层组),以及心脏移植供体的主动脉壁标本30例(对照组)。用苏木素-伊红(HE)染色和弹性纤维(EVG)染色的方法分析两组主动脉壁组织结构的差异。进一步通过蛋白质免疫印迹(WB),免疫荧光(IF)和免疫组化(IHC)检测SAP在主动脉壁组织中的表达水平及亚细胞定位。结果HE和EVG染色结果显示,与对照组相比,夹层组主动脉平滑肌细胞排列紊乱、弹性纤维断裂且减少。蛋白质免疫印迹、免疫荧光及免疫组化实验显示,与对照组对比,SAP蛋白表达水平在夹层组主动脉壁组织中显著降低(P<0.05),且免疫荧光染色显示SAP蛋白主要定位于细胞质和细胞外基质中。结论与对照组相比,主动脉夹层患者的主动脉壁组织中SAP蛋白水平降低,提示SAP蛋白可能与主动脉夹层的发生发展相关。

Objective To investigate the expression level of serum amyloid P component(SAP)in aorta of patients with aortic dissection and its potential clinical significance.Methods A total of 60 aortae samples were collected from patients with Stanford type A aortic dissection(TAAD)and 30 normal aortae samples were collected from heart transplant donors from January 2020 to December 2021 in the Division of Cardiovascular Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.The differences in the aortic wall morphology between the two groups were analyzed by hematoxylin-eosin(HE)staining,and elastic fiber(EVG)staining.The subcellular localization and the expression level of SAP in the aortic wall tissues were further examined by Western blotting,immunofluorescence staining and immunohistochemistry.Results HE and EVG staining showed that the aortic smooth muscle cells were disorganized and elastic fibers were broken and reduced in the aorta of patients with TAAD compared with the control group.The results of Western blotting,immunofluorescence staining and immunohistochemistry staining showed that the expression level of SAP protein was significantly reduced in the aortic wall tissue of patients with TAAD compared with the control group(P<0.05),and the results of immunofluorescence staining showed that SAP protein was mainly localized in the cytoplasm and extracellular matrix.Conclusions SAP protein reduces in the aortic wall tissues of aortic dissection patients,suggesting that SAP protein may be associated with the development of aortic dissection.