口服太子参皂苷对鸡免疫功能和炎症反应的影响

Effects of oral administration of Pseudostellaria heterophylla saponins on immune function and inflammatory response in chickens

【目的】旨在研究口服太子参皂苷(RP)对鸡免疫功能和炎症反应的影响,为使用RP作为免疫增强剂提供科学依据,对家禽养殖业的疾病预防和健康维护具有实际应用价值。【方法】试验以鸡毒支原体(MG)抗原、抗体均为阴性的30日龄健康河田鸡为研究对象,根据初始体质量平均分为5组,空白组不给药及疫苗,疫苗对照组口服灭菌生理盐水7 d后免疫MG弱毒疫苗,100,20,5 mg/kg RP试验组分别连续口服RP 7 d后免疫MG弱毒疫苗,一免后28 d进行二免,试验期49 d。采用间接ELISA法检测血清中MG特异性抗体消长规律及抗体阳性率和细胞因子(IFN-γ、IL-4、IL-5、IL-1α、CCL4、IL-17A、IL-6)含量。【结果】(1)RP组与疫苗对照组相比,口服RP能有效提高一免及二免后7 d的MG特异性抗体滴度及抗体阳性率(P<0.05),其中以RP高剂量(100 mg/kg)效果最佳。(2)细胞因子表达水平中,与疫苗对照组相比,RP高剂量(100 mg/kg)和中剂量(20 mg/kg)组血清中免疫因子(IFN-γ、IL-4、IL-5)的含量在一免后3 d呈现显著上升(P<0.05),一免后7 d无明显差异;(3)与疫苗对照组相比,RP组炎症细胞因子(IL-1α、CCL4、IL-17A、IL-6)血清中的含量显著降低(P<0.05),高剂量组(100 mg/kg)下降最为显著。【结论】首免前口服太子参皂苷(RP)能够提高河田鸡的免疫功能,并降低由MG弱毒疫苗引起的炎症反应,其中100 mg/kg RP效果最佳。

[Objective]The aim of this study is to investigate the effects of oral administration of Radix Pseudostellariae(RP)on immune function and inflammatory response in chickens.By providing a scientific basis for the use of RP as an immunopotentiator,this study has practical application value for disease prevention and health maintenance in poultry industry.[Method]The 30-day-old healthy Hetian chickens with negative Mycoplasma gallisepticum(MG)antigen and antibody were used as subjects in the study.According to the initial body weight,they were divided into five groups,the blank group was not administered with drugs and vaccines.The vaccine control group was immunized with MG attenuated vaccine after oral administration of sterilized normal saline for 7 days.The three dose RP groups were orally administered with RP(100 mg/kg,20 mg/kg,5 mg/kg)for 7 days and then immunized with MG attenuated vaccine,the second immunization was conducted 28 days after the first immunization,and the test period was 49 days.Indirect ELISA was used to detect the growth and decline of MG-specific antibodies in serum,the positive rate of antibodies and the content of cytokines(IFN-γ,IL-4,IL-5,IL-1α,CCL4,IL-17A,IL-6).[Result](1)Compared with the vaccine control group,oral administration of RP before immunization can effectively improve the MG specific antibody titer and antibody positive rate 7 days after the first immunization and the second immunization.Among the three doses,the high dose of RP(100 mg/kg)had the best effect.(2)In the expression level of cytokines,compared with the vaccine control group,the content of immune factors(IFN-γ,IL-4,IL-5)in the serum of the RP high-dose(100 mg/kg)and medium-dose(20 mg/kg)groups increased significantly on the 3rd day after the first immunization(P<0.05),and there was no significant difference on the 7th day after the first immunization.(3)Compared with the vaccine control group,the serum levels of inflammatory cytokines(IL-1α,CCL4,IL-17A,IL-6)in the RP group were significantly decreased(P<0.05),and the high-dose group(100 mg/kg)decreased most significantly.[Conclusion]The results shows that oral administration of RP before immunization could improve the immune function of Hetian chickens and reduce the inflammatory response caused by MG attenuated vaccine,and the dose of RP 100 mg/kg has the best effect.