水苏糖对秦川牛精液冷冻保存效果的影响

Effect of Stachyose on the Cryopreservation of Qinchuan Bull Semen

试验主要探究在牛精液冷冻稀释液中添加水苏糖对秦川牛精液冷冻保存效果的影响。添加不同浓度(0、20、40、60、80 mg/mL)水苏糖到牛精液冷冻基础稀释液中,检测冷冻-解冻后秦川牛精子运动性能、质膜完整性、顶体完整率、线粒体活性及抗氧化性相关指标。结果表明,与对照组相比,40 mg/mL水苏糖处理显著(P<0.05)提高冷冻-解冻后精子活力(43.83%)和顶体完整率(78.70%);质膜的完整性和线粒体活性在随着水苏糖浓度的增加显著提升,但质膜的完整性在不同水苏糖浓度之间差异不显著(P>0.05);同时,40 mg/mL水苏糖组显著提高GSH-Px酶和CAT酶的活性(P<0.05),并显著降低精子细胞中的MDA含量(P<0.05)。综上表明,40 mg/mL水苏糖处理显著提升冷冻-解冻后牛精子品质和抗氧化能力,对秦川牛的冷冻精液具有较好保存效果。

The experiment was to explore the effect of stachyose on semen cryopreservation of Qinchuan bull.Different concentrations of stachyose(0 mg/mL,20 mg/mL,40 mg/mL,60 mg/mL and 80 mg/ml)were supplemented to the frozen diluent of bull semen,then the sperm motility,membrane integrity,acrosome integrity,mitochondrial activity and antioxidant properties related indicators of frozen-thawed semen in Qinchuan bull were tested.The results showed that 40 mg/mL stachyose treatment significantly(P<0.05)improved sperm motility(43.83%)and acrosome integrity(78.70%)after freezing-thawing,compared with the control group;membrane integrity and mitochondrial activity were enhanced significantly with the increase of stachyose concentration(P<0.05),but the membrane integrity was not significantly different between the different concentrations of stachyose(P>0.05);in addition,the activities of GSH-Px enzyme and CAT enzyme were significantly increased(P<0.05),and the content of MDA significantly reduced(P<0.05)in the 40 mg/mL stachyose.In summary,the 40 mg/mL stachyose treatment significantly improves the quality and antioxidant capacity of freezing-thawing semen,and has a good preservation effect on the frozen semen in Qinchuan bull.