半干旱区2种雪茄烟叶发酵后差异代谢物分析

Differential metabolites analysis on two varieties of fermented cigar leaves in semi-arid region

通过比较2个雪茄品种‘海研103’和‘青雪204’发酵后烟叶的代谢产物,探究不同品种烟叶间代谢组的差异。采用超高效液相色谱-四极杆飞行时间质谱联用技术(UHPLC-QTOF-MS),对2个雪茄品种烟叶化学成分进行了非靶向代谢组学分析。结果表明:‘海研103’和‘青雪204’的代谢物相对含量存在明显差异。2个雪茄品种烟叶共筛选鉴定出179个差异代谢物,‘海研103’较‘青雪204’有114个代谢物显著上调,有65个显著下调。对前40个主要差异代谢物用PLS-DA法进行分析,鉴定为有机酸10种、氨基酸7种、酚酸类4种、核苷酸及类似物4种、碳水化合物3种、黄酮类3种、木脂素和香豆素3种,其他6种。结合KEGG通路分析,发现12条2个品种显著富集的代谢通路。

By comparing the metabolites between two varieties of fermented cigar leaves‘Haiyan 103’and‘Qingxue 204’,the metabolome differences between two varieties of cigar leaves were explored.In the study,non-targeted metabolomic analysis of the chemical components of two fermented cigar leaves were analyzed by Ultra High-Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometric(UHPLC-QTOF-MS).The results indicated that,the metabolomes relative content of‘Haiyan 103’and‘Qingxue 204’were significantly different.A total of 179 differential metabolites were identified in two varieties of fermented cigar leaves,of which 114 metabolites were significantly up-regulated in‘Haiyan 103’compared to‘Qingxue 204’,while 65 were significantly down-regulated.Forty major differential metabolites were qualitatively and quantitatively identified,including 10 organic acids,7 amino acids,4 phenolic acids,4 nucleotides and analogues,3 carbohydrates,3 flavonoids,3 lignans and coumarins,and 6 others.Based on KEGG pathway analysis,12 significantly enriched pathways were identified.