鬼箭羽对糖尿病肾病小鼠肾脏结构及功能的影响

Effects to Euonymus alatus on renal structure and function of diabetic nephropathy mice

目的:探讨鬼箭羽对糖尿病肾病小鼠肾脏形态结构及功能的影响。方法:10只7周龄db/m小鼠为空白组;60只db/db小鼠随机分为模型组,鬼箭羽低、中、高剂量[1.5、3.0、6.0 g/(kg·d)]组和厄贝沙坦[20 mg/(kg·d)]组,每组12只;空白组和模型组灌胃生理盐水,余4组分别灌胃相应药物,连续给药12周。实验期间观察小鼠一般状态,给药前后检测空腹血糖、24 h尿量和饮水量。末次给药后检测尿白蛋白/肌酐(UACR)、血肌酐(SCr)、血尿素氮(BUN);处死小鼠,称重双肾,计算肾脏指数(KI);取左肾组织,HE、PAS、Masson染色,观察组织学表现;分离右肾皮质,透射电镜下观察超微结构。结果:与模型组比较,鬼箭羽高剂量组及厄贝沙坦组小鼠KI、空腹血糖、SCr、BUN、UACR均降低(P均<0.05),系膜基质百分比及胶原面积百分比降低(P<0.05),肾脏病理损害减轻,肾脏基底膜厚度减少(P<0.05),足突融合改善。结论:高剂量鬼箭羽可有效降低糖尿病肾病小鼠的血糖,修复肾脏功能及结构,对糖尿病肾病有较好的治疗作用。

Aim:To investigate the effects of Euonymus alatus(GJY)on renal structure and function of mice with diabetic nephropathy.Methods:Ten 7-week-old db/m mice were used as blank group;60 db/db mice were randomly allocated into model group,GJY low,medium,high dose[1.5,3.0,6.0 g/(kg·d)]groups and irbesartan[20 mg/(kg·d)]group,with 12 mice in each group.The blank group and model group received intragastric administration of normal saline,while the other 4 groups received corresponding drugs for 12 weeks.During the experiment,the general state of mice was observed,and the fasting blood glucose(FBG),24-hour urine output and water intake were detected before and after administration.After the last administration,urine albumin/creatinine(UACR),serum creatinine(SCr)and blood urea nitrogen(BUN)were measured;the kidney were weighed to calculate the kidney index(KI),the left kidney tissue samples were stained with HE,PAS and Masson,and the histological findings were observed under optical microscope;the right renal cortex was isolated and the ultrastructural changes were observed under transmission electron microscope.ResuIts:Compared with model group,KI,FBG,SCr,BUN and UACR of mice in GJY high dose group and irbesartan group decreased(P<0.05),mesangial matrix percentage and collagen area percentage decreased(P<0.05),renal pathological damage was alleviated,the thickness of kidney basement membrane decreased(P<0.05),and the fusion of foot process improved.ConcIusion:High dose of GJY can effectively reduce blood sugar levels,repair kidney function and structure,and has good therapeutic effects on diabetic nephropathy.