GIPR表达与胃癌临床病理特征和免疫浸润的关系

Relationship of GIPR expression with clinicopathological features and immune infiltration in gastric cancer tissues

目的探讨葡萄糖依赖性促胰岛素多肽受体(GIPR)表达与胃癌临床病理特征和免疫浸润的关系。方法收集2017年3月至2018年4月于本院手术的胃癌患者61例,采用实时定量PCR检测胃癌组织的GIPR表达水平,结合临床病理资料和随访数据分析GIPR表达在胃癌中的临床意义,使用肿瘤免疫估计资源(TIMER)评估GIPR表达与免疫细胞浸润间的关联性。结果与癌旁正常组织相比,胃癌组织中GIPR表达升高,且与肿瘤大小、TNM分期和淋巴结转移有关(P<0.05);GIPR高表达者的中位无病生存期为43.0(95%CI:37.0~45.5)个月,低于低表达者的52.0(95%CI:49.5~60.0)个月(HR=2.625,95%CI:1.965~4.652,P=0.004),多因素分析显示GIPR高表达是胃癌患者预后不良的独立因子(HR=2.522,95%CI:1.530~4.157,P=0.009)。TIMER数据库分析结果显示GIPR在胃癌中与B细胞浸润水平呈正相关(r=0.172,P<0.001),而与CD8+T细胞(r=-0.003,P=0.949)、CD4+T细胞(r=0.062,P=0.234)、巨噬细胞(r=-0.019,P=0.711)、中性粒细胞(r=0.034,P=0.515)和树突状细胞(r=0.043,P=0.406)的免疫浸润水平无相关性。结论GIPR在胃癌组织中异常高表达,且与不良预后和B细胞浸润有关,参与了胃癌的恶性进展过程。

Objective To explore the relationship of glucose-dependent insulinotropic polypeptide receptor(GIPR)expression with clinicopathological features and immune infiltration in gastric cancer tissues.Methods Sixty-one gastric cancer patients who underwent surgery in our hospital from March 2017 to April 2018 were collected.GIPR expression in gastric cancer tissues was detected by real-time quantitative PCR.Clinical pathological data and follow-up data were combined to analyze the clinical significance of GIPR expression in gastric cancer.Tumor immune estimation resources(TIMER)were used to evaluate the association between GIPR expression and immune cell infiltration.Results Compared with normal tissues adjacent to cancer,the expression of GIPR in gastric cancer tissues was significantly increased,and the expression of GIPR in gastric cancer tissues was related to tumor size,TNM stage,and lymph node metastasis(P<0.05).The median disease-free survival of patients with high expression of GIPR was 43.0(95%CI:37.0-45.5)months,which was lower than the 52.0(95%CI:49.5-60.0)months of the low expression individuals(HR=2.625,95%CI:1.965-4.652,P=0.004).Multivariate analysis showed that high expression of GIPR was an independent factor for poor prognosis in gastric cancer patients(HR=2.522,95%CI:1.530-4.157,P=0.009).The TIMER database analysis results showed that GIPR was positively correlated with B cell infiltration levels in gastric cancer(r=0.172,P<0.001),but not with immune infiltration levels of CD8+T cells(r=-0.003,P=0.949),CD4+T cells(r=0.062,P=0.234),macrophages(r=-0.019,P=0.711),neutrophils(r=0.034,P=0.515),and dendritic cells(r=0.043,P=0.406).Conclusion GIPR is abnormally highly expressed in gastric cancer tissue and is associated with poor prognosis and B cell infiltration,participating in the malignant progression of gastric cancer.