宫颈癌组织中GLRX的表达及临床意义

Expression and clinical significance of GLRX in cervical cancer tissues

目的探讨谷氧还蛋白(GLRX)在宫颈癌组织中的表达及其预后意义。方法采用实时定量PCR检测120对宫颈癌组织和癌旁正常组织中GLRX的表达水平,分层分析组织GLRX水平与宫颈癌患者临床病理特征和预后的关系,Cox比例风险模型进行风险因素分析。结果宫颈癌组织的GLRX表达水平为2.44±0.86,高于癌旁正常组织的1.00±0.65(P<0.05);组织GLRX表达水平预测宫颈癌发生的曲线下面积为0.853(95%CI:0.788~0.919)。宫颈癌组织GLRX表达与患者的肿瘤FIGO分期、淋巴结转移、肿瘤浸润以及肿瘤最大径有关(P<0.05)。单因素分析显示GLRX表达与宫颈癌的总生存期(OS)有关,其中低表达组的中位OS未达,优于高表达组的40.0个月(P<0.05);多因素因素分析显示GLRX低表达是影响宫颈癌患者预后的独立保护因素(HR=0.215,95%CI:0.095~0.486,P<0.001)。结论GLRX过表达参与了宫颈癌的恶性进展,可作为宫颈癌患者诊断及预后预测的潜在分子标志物。

Objective To explore the expression and prognostic significance of glutaredoxin(GLRX)in cervical cancer tissues.Methods Real-time quantitative PCR was used to detect the expression level of GLRX in 120 cases of cervical cancer tissues and normal adjacent tissues.Relationship of GLRX levels in tissues with clinicopathological characteristics and prognosis of cervical cancer patients were analyzed in a hierarchical manner,and Cox proportional risk model was employed for risk factor analysis.Results The expression level of GLRX in cervical cancer tissues was 2.44±0.86,higher than 1.00±0.65 in normal adjacent tissues(P<0.05).The area under the curve for predicting the occurrence of cervical cancer based on the expression level of GLRX in tissues was 0.853(95%CI:0.788-0.919).GLRX expression in cervical cancer patients was correlated with FIGO stage,lymph node metastasis,tumor infiltration and maximum tumor diameter(P<0.05).Univariate analysis showed that GLRX expression was associated with overall survival(OS)in cervical cancer,with the median OS not reached in the low expression group,which was better than 40.0 months in the high expression group(P<0.05).Multivariate analysis showed that low expression of GLRX was an independent protective factor affecting the prognosis of cervical cancer patients(HR=0.215,95%CI:0.095-0.486,P<0.001).Conclusion GLRX overexpression can serve as a potential molecular marker for the diagnosis and prognosis of cervical cancer patients.