葱间作对连作葡萄植株生长的转录组学分析

Transcriptomic Analysis of Intercropping with Scallion on the Growth of Replanting Grapevine

为从分子水平上揭示葱缓解葡萄连作障碍的成因,以葡萄砧木‘贝达’扦插苗为试材,间作四季小葱(Allium fistulosum L)。采用Illumina高通量测序平台进行葡萄根尖的转录组测序,共得到38.74 Gb Clean data,葱间作后葡萄根尖共有285个基因表现出差异表达,其中上调基因为161个,下调基因为124个。KEGG分析结果表明,差异基因主要分布在植物激素信号转导途径、淀粉和蔗糖代谢途径、谷胱甘肽代谢途径、植物病原互作等。其中IAA、BAK1、SUS、GST、RPS4、PBS1基因及PIF3、ARR、WRKY52转录因子上调表达可能与间作后葡萄植株长势增强有关。研究结果为抗性基因的挖掘和筛选奠定了理论基础。

To investigate how scallion(Alliumfistulosum L)can help overcome replant disease in grapevine at the molecular level,an experiment was conducted using'Beta'(Vitisriparia×V.labrusca)rootstock cutting seedlings as test materials and interplanted them with scallion.The transcriptome of grape root tips was sequenced by Illumina high throughput sequencing platform,and a total of 38.74Gb of Clean data were obtained.A total of 285 genes in grapevine root tips showed differential expression after intercropping with scallion,among which 161 up-regulated genes and 124 down-regulated genes.KEGG analysis results showed that the differential genes were mainly distributed in plant hormone signal transduction,starch and sucrose metabolism,and glutathione metabolism,plant-pathogen interaction,etc.Among them,the up-regulated expression of IAA,BAK1,SUS,GST,RPS4,PBS1 and PIF3,ARR,WRKY52 transcription factors may be related to the enhanced growth of grapevine seedlings after intercropping.The research results can provide a theoretical foundation for the excavating and screening of resistance genes.