Bdfor对橘小实蝇联系性颜色学习的影响

Effects of Bdfor on associative color learning of Bactrocera dorsalis

【目的】从橘小实蝇中鉴定Bdfor基因,研究其对橘小实蝇联系性颜色学习的影响,解析昆虫联系性颜色学习的内在机制。【方法】根据黑腹果蝇Dmfor基因,结合橘小实蝇基因组和NCBI数据库,筛选出橘小实蝇Bdfor基因序列,并进行生物信息学分析和克隆测序验证;同时,对橘小实蝇进行联系性颜色学习试验,利用RNAi技术靶向沉默Bdfor基因后,观察其对橘小实蝇联系性颜色学习的影响。【结果】成功筛选出橘小实蝇Bdfor,其编码的for蛋白与3种实蝇科昆虫辣椒实蝇、橄榄实蝇、地中海实蝇的for蛋白聚为一族,含有1个STKs结构域和2个CAP-ED结构域。此外,橘小实蝇具备一定的联系性学习能力,当以绿色或蓝色为条件刺激和蔗糖配对,进行奖励学习时,橘小实蝇可将绿色或蓝色和蔗糖相联系,进行颜色学习,改变自身趋性。当以黄色为条件刺激和奎宁配对,进行惩罚学习时,橘小实蝇也可将黄色和奎宁相联系,同样进行颜色学习并改变趋性。然而,在靶向沉默Bdfor后,这种学习能力便消失或减弱。【结论】Bdfor影响橘小实蝇联系性颜色学习行为,这一结果为进一步开发害虫防治新技术提供了依据。

【Aim】In this study,the Bdfor was identified to further explore the effects on associative color learning of Bactrocera dorsalis.【Method】Based on Drosophila melanogaster gene Dmfor,the Bdfor was screened out from B.dorsalis genomics and transcriptomics database in NCBI and then performed bioinformatics analysis.Meantime,the behavioral bioassay on associative color learning of B.dorsalis adults as well as RNAi silence experiments were carried out.【Result】The B.dorsalis Bdfor,which contains a STKs protein domain and two CAP-ED protein domains,was screened out successfully and the amino acid sequence of this gene was similar with that of B.latifrons,B.oleae,and Ceratitis capitata.In addition,the results showed that B.dorsalis has the ability of associative color learning.After pairing green or blue with sucrose,and yellow with water in petri dishes for reward learning,the tendency of B.dorsalis to green or blue was significantly increased.Meanwhile,pairing yellow with quinine and green or blue with water in petri dishes for punitive learning,the yellow preference of B.dorsalis decreased significantly.However,this ability of associative color0x0E䥺Symbol`@@0x0F learning in B.dorsalis was significantly reduced or disappeared by RNAi experiments.【Conclusion】Silencing Bdfor impaired the color learning in the flies which offers evidence for developing new management against insect pests.