CMIP促进人结直肠癌对阿霉素耐药性的机制研究

Mechanism of CMIP promoting drug resistance to doxorubicin in human colon cancer

目的探讨CMIP对人结直肠癌阿霉素耐药性的影响。方法收集淮北市人民医院2021年1月—2022年12月60例结直肠癌患者的癌组织标本和癌旁正常组织标本,免疫组化检测CMIP在结直肠癌组织中的表达,分析CMIP与结直肠癌临床病理特征的相关性。采用CCK8实验检测人结直肠癌HCT116、HT29细胞株的细胞增殖活力,并采用细胞划痕实验检测细胞迁移能力。运用catRAPID omics v2.0网站预测CMIP的潜在互作靶点,通过Metascape网站对CMIP的靶基因进行GO功能和KEGG通路富集分析。结果CMIP在结直肠癌组织中呈高表达,CMIP表达与结直肠癌临床病理特征存在相关性。过表达/敲低CMIP可促进/抑制结直肠癌细胞的增殖和迁移,过表达/敲低CMIP可促进/抵抗结直肠癌阿霉素耐药。通过catRAPID omics v2.0数据库共发现205个CMIP相关mRNA,GO和KEGG分析结果显示,CMIP可能通过多种生物学过程和信号通路介导结直肠癌阿霉素耐药。结论CMIP可促进结直肠癌的发展和阿霉素耐药,具体作用机制还需进一步研究。

Objective To investigate the effects of C-Maf inducing protein(CMIP)on doxorubicin resistance in human colorectal cancer.Methods Sixty cases of colorectal cancer tissues and 60 cases of adjacent normal tissues were collected from Huaibei People's Hospital from January 2021 to December 2022.The expression of CMIP in colorectal cancer tissues was detected by immunohistochemistry,and the correlation between CMIP and clinicopathological characteristics of colorectal cancer was analyzed.Proliferation and migration of human colorectal cancer HCT116 and HT29 cell lines were assessed using CCK8 assay and cell scratch assay,respectively.Potential interacting targets of CMIP were predicted using the catRAPID omics v2.0 website.The GO function and KEGG pathway enrichment analysis of the target genes of CMIP were performed by Metascape website.Results The expression of CMIP was upregulated in human colorectal cancer tissues,and was correlated with the clinicopathological features of colorectal cancer.Overexpression/knockdown of CMIP promoted/inhibited proliferation and migration of colorectal cancer cells,as well as enhanced/resisted adriamycin resistance in these cells.A total of 205 CMIP-related mRNAs were found by catRAPID omics v2.0 database.GO and KEGG analysis results indicated that CMIP may mediate doxorubicin resistance in colorectal cancer through multiple biological processes and signaling pathways.Conclusion CMIP can facilitate the development of colorectal cancer and its resistance to doxorubicin.However,further investigation is required to elucidate its specific mechanism.