红树莓GeBP基因家族鉴定及表达分析

Identification and expression analysis of GeBP gene family in red raspberry

无表皮毛增强子结合蛋白(GeBP)是植物体内一种特有的蛋白,在植物生长发育、激素水平和逆境胁迫中发挥重要作用。本研究以‘海尔特兹’红树莓为材料,通过转录组测序、生物信息学分析、qRTPCR技术,对GeBP基因家族成员进行了鉴定和表达模式研究。结果显示:GeBP基因家族3个成员的蛋白质氨基酸数量在236~407个之间,分子质量在26.66~42.25 kDa之间,等电点在4.84~9.52之间。RuGeBP-1和RuGeBP-2为不稳定存在的蛋白质,RuGeBP-3为稳定存在的蛋白质。三者均为亲水性蛋白质、含有最多的氨基酸为丝氨酸、均无信号肽、为非分泌蛋白、均定位于细胞核、均为非跨膜蛋白、含有DUF573保守结构域,二级结构均由α螺旋、β转角、无规则卷曲、折叠延伸链共4种结构构成,且与三级结构预测结果一致。RuGeBP基因家族有0~2个内含子和6个重要的保守基序;系统进化分析表明红树莓与草莓的亲缘关系更近。启动子顺式作用元件表明RuGeBP基因家族成员主要参与激素响应、防御和应激响应、植物生长发育。RuGeBP基因家族存在组织特异性表达,主要在果实发育前期和根中表达。RuGeBP-1、RuGeBP-3可能与果实中脱落酸等激素响应机制以及根的生长有关,RuGeBP-2可能与根的生长和防御机制有关。

Glabrous-enbancer binding protein(GeBP)is a unique protein in plants,which plays an important role in plant growth and development,hormone levels and stress.In this study,using'Heritage'red raspberries as materials,the GeBP gene family members were identified and their expression patterns were studied by transcriptome sequencing,bioinformatics analysis and qRT-PCR.The results showed that:three members of the GeBP gene family the protein amino acid number ranged from 236–407,the molecular weight ranged from 26.66–42.25 kDa,and the isoelectric point ranged from 4.84–9.52.RuGeBP-1 and Ru-GeBP-2 are unstable proteins,and RuGeBP-3 is stable.All three are hydrophilic proteins,contain most amino acids as serine,have no signal peptide,are non-secreted proteins,are localized in the nucleus,are non-transmembrane proteins,contain DUF573 conserved domain,secondary structure prediction showed that the proteins encoded by RuGeBPs were composed ofαhelix,βcorner,random curling and folding extension chains,and the tertiary structure prediction results were consistent with the secondary structure.The analysis of gene structure and conserved motifs showed that RuGeBP gene family had 0-2 introns,and have six important conserved motifs;Phylogenetic analysis showed that red raspberry and strawberry were more closely related.The promoter cis-acting elements indicated that RuGeBP gene family was mainly involved in hormone response,defense and stress response,plant growth and development.RuGeBP gene family had tissue-specific expression,which was mainly expressed in early fruit development and roots.Ru-GeBP-1,RuGeBP-3 might be related to the response mechanism of abscisic acidand other hormones in fruit and the growth a of roots,RuGeBP-2 may be involved in root growth and defense mechanisms.