摘要
1-戊烯-3-酮(1-penten-3-one)作为挥发性气体能够诱导拟南芥叶肉细胞早期信号的产生和抗虫性的增加。为了探究质膜NADPH氧化酶在1-戊烯-3-酮诱导的拟南芥抗虫防御反应中的作用,本研究以野生型拟南芥和2种拟南芥质膜NADPH氧化酶的突变体(Atrbohd和Atrbohf)为实验材料,通过生物学实验的测定和实时定量荧光PCR技术研究1-戊烯-3-酮诱导的野生型拟南芥与突变体不同的抗虫性。结果表明,用1-戊烯-3-酮熏蒸可以诱导拟南芥NADPH氧化酶基因的表达,NADPH氧化酶通过影响小菜蛾的取食行为来提高拟南芥抵御虫害的能力,并参与1-戊烯-3-酮熏蒸诱导的拟南芥防御基因Thi2.1、VSP1、PDF1.2和VSP2的表达。本研究以期为揭示NADPH氧化酶在1-戊烯-3-酮诱导拟南芥抗虫性增强过程中的分子机制提供一些理论依据。
As a volatile gas,ethyl vinyl ketone(1-penten-3-one)can trigger early signal generation and boost the insect resistance of Arabidopsis thaliana mesophyll cells.To investigate the role of plasma membrane NADPH oxidase in insect resistance in Arabidopsis induced by 1-penten-3-one,biological tests and real-time quantitative PCR were performed in WT Arabidopsis and two mutants of NADPH oxidase in plasma membrane(Atrbohd and Atrbohf).The results showed that fumigation with 1-pentene-3-one could stimulate the expression of NADPH oxidase gene in Arabidopsis,and NADPH oxidase may enhance Arabidopsis's capacity to resist insect pests by affecting the eating habits of Plutella xylostella.Additionally,NADPH oxidase contributed to the expression of the defense genes Thi2.1,VSP1,PDF1.2,and VSP2 in Arabidopsis,which are stimulated by 1-pentene-3-one fumigation.This study aims to provide theoretical support for the molecular mechanism of NADPH oxidase in increasing insect resistance in Arabidopsis induced by 1-pentene-3-one.
作者
张笑
丁玉勤
季亚溪
沈应柏
ZHANG Xiao;DING Yuqin;JI Yaxi;SHEN Yingbai(College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong,Shanxi 030619,China;College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;Hebei Tangshan Foreign Language School,Tangshan,Hebei 063000,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第6期1027-1034,共8页
Plant Physiology Journal
基金
山西省基础研究计划(202203021222242)
山西高等学校科技创新项目(2019L0802)
河北省教育科学“十四五”规划课题(2205240)
河北省教育装备“十四五”规划课题(JYZB2204124)。
