化学发光法与乙肝病毒DNA荧光定量在乙肝检测中的应用价值

Application Value of Chemiluminescence Assay and Hepatitis B Virus DNA Fluorescence Quantification in the Detection of Hepatitis B

目的:明确化学发光法(CLIA)、乙肝病毒DNA荧光定量技术在乙肝检测中的价值。方法:选取285例乙肝患者和280例肝硬化患者为检测对象,分别对样本进行CLIA、乙肝病毒DNA荧光定量分析,统计相关检测数据。结果:乙肝组HBsAg、HBsAb、HBeAg、HBeAb、HBcAb水平均明显低于肝硬化组(P<0.05),HBV-DNA水平明显高于肝硬化组,随着HBV-DNA水平上升,乙肝患者的肝功能指标均有不同程度提高(P<0.05)。经Pearson相关性分析,HBV-DNA和HBsAg、HBsAb、HBeAg、HBeAb、HBcAb均为负相关(t=-0.876、-0.956、-0.974、-0.922、-0.981)。结论:CILA、乙肝病毒DNA荧光定量检测技术在乙肝检测中都有一定价值,两者之间为负相关,可为乙肝诊断、预后评估提供参考信息。

Objective:To clarify the value of chemiluminescence immunoassay(CLIA)and hepatitis B virus(HBV)DNA fluorescence quantification in the detection of hepatitis B.Methods:A total of 285 patients with hepatitis B and 280 patients with liver cirrhosis were selected as subjects.Samples from both groups were analyzed using CLIA and HBV DNA fluorescence quantification,with relevant testing data compiled and statistically analyzed.Results:Levels of HBsAg,HBsAb,HBeAg,HBeAb,and HBcAb were significantly lower in the hepatitis B group compared to the liver cirrhosis group(P<0.05),whereas HBV-DNA levels were sign-ificantly higher.As HBV-DNA levels increased,various liver function indicators for hepatitis B patients also showed corresponding elevations to varying degrees(P<0.05).Pearson correlation analysis revealed negative correlations between HBV-DNA and HBsAg,HBsAb,HBeAg,HBeAb,and HBcAb(t=-0.876,-0.956,-0.974,-0.922,-0.981,respectively).Conclusion:Both CLIA and HBV DNA fluorescence quantification techniques demonstrate value in detecting hepatitis B,showing a negative correlation between them.These methods can provide reference information for the diagnosis and prognosis assessment of hepatitis B.