西瓜皮多糖对鲤免疫调节作用的初步研究

Extraction of polysaccharide from melon peel and its effect on immune regulation of Cyprinus carpio

本文旨在研究西瓜皮多糖(Watermelon peel polysaccharide,WP)对鲤(Cyprinus carpio)的体外免疫调节功能。将提取的不同浓度的WP(250μg/mL、500μg/mL、1000μg/mL)与鲤头肾单核细胞共同培养,采用中性红摄入法和MTT比色法测定不同浓度的WP对鲤头肾单核细胞的增殖能力及吞噬活性的影响,并通过对细胞因子(TNF-α、IL-10、IL-1β、TGF-β)含量的测定以及一氧化氮(NO)含量和碱性磷酸酶(AKP)活性的检测,评估WP对鲤的免疫调节作用。研究结果显示,在一定浓度范围内,WP可促进鲤头肾细胞的增殖能力和吞噬活性,并显著上调细胞因子和NO的含量,以及增强AKP活性。结果表明,WP对鲤具有免疫调节作用,WP可作为饲料添加剂用于提高鲤的免疫力。

The aim of this study was to investigate the immunomodulatory effects of Watermelon peel polysac-charide(WP)on Cyprinus carpio.In this study,different concentrations of extracted WP(250μg/mL,500μg/mL,1000μg/mL)were co-cultured with head kidney monocytes.Neutral red intake method and MTT colorimetry were used to determine the effects of different concentrations of WP on the proliferation and phagocytic activity of head kidney monocytes.Cytokines(TNF-α,IL-10,IL-1β,TGF-β),nitric oxide(NO)content and alkaline phosphatase(AKP)activity were measured to evaluate the immunomodulatory effects of WP.The results showed that WP could promote the proliferation capacity and phagocytosis activity of head kidney cells,significantly up-regulate the con-tents of cytokines and NO,and enhance the activity of AKP in a certain concentration range.The results showed that WP had immunomodulatory effects on common carp,and WP could be used as a feed additive to improve the immunity of common carp.