基于果蝇模型建立的菊苣提取物抗尿酸盐沉积作用研究

Anti-urate Deposition Effects of Cichorium intybus L.Extract Based on Drosophila Model

目的:建立尿酸盐沉积果蝇模型并确定其造模条件,并初步观察菊苣提取物抗尿酸盐沉积的作用。方法:以5%酵母提取物饲喂诱导果蝇尿酸盐沉积,通过果蝇马氏小管及后肠偏振光成像、六胺银染色及其匀浆液尿酸水平评价果蝇尿酸盐沉积状态,考察雌雄性别、不同造模时间对果蝇尿酸盐沉积的影响,并应用该模型研究菊苣提取物抗尿酸盐沉积的作用。结果:5%酵母提取物饲喂可成功诱导果蝇尿酸盐沉积;雄性果蝇在造模3 d后开始出现尿酸盐沉积,在造模7 d后大部分出现尿酸盐沉积,各造模时间点果蝇马氏小管及后肠匀浆液尿酸水平持续升高,各造模时间点之间差异不明显;雌性果蝇在造模10 d后开始出现尿酸盐沉积,在造模14 d后大部分出现尿酸盐沉积,各造模时间点果蝇马氏小管及后肠匀浆液尿酸水平持续升高,马氏小管及后肠匀浆液尿酸水平持续升高,各造模时间点之间比较,差异有统计学意义(P<0.001);菊苣提取物可减少果蝇马氏小管及后肠偏振光信号,并明显降低其匀浆液的尿酸水平(P<0.001)。结论:以5%酵母提取物饲喂雌性果蝇14 d可较稳定诱导尿酸盐沉积,菊苣提取物可在果蝇模式动物上发挥抗尿酸盐沉积的作用。

Objective:To establish a drosophila model of urate deposition,determine modeling conditions,and preliminarily observe the anti-urate deposition effects of Cichorium intybus L.extract.Methods:Drosophila were fed a 5% yeast extract diet to induce urate deposition.The urate deposition status was evaluated using polarized light imaging of the Malpighian tubules and hindgut,hexamine silver staining,and measurement of uric acid levels in homogenates.The effects of gender and different modeling times on urate deposition were investigated.This model was then used to study the anti-urate deposition effects of C.intybus L.extract.Results:Feeding drosophila with 5% yeast extract successfully induced urate deposition.Male drosophila began to show urate deposition after 3 days of modeling,with most showing urate deposition after 7 days.The uric acid levels in the Malpighian tubules and hindgut homogenates of male drosophila continuously increased over time,with no significant differences between different time points.Female drosophila began to show urate deposition after 10 days of modeling,with most showing urate deposition after 14 days.The uric acid levels in the Malpighian tubules and hindgut homogenates of female drosophila continuously increased over time,with significant differences between different time points(P<0.001).C.intybus L.extract reduced the polarized light signal in the Malpighian tubules and hindgut of drosophila and significantly lowered uric acid levels in homogenates(P<0.001).Conclusion:Feeding female drosophila with 5% yeast extract for 14 days can reliably induce urate deposition.C.intybus L.extract can exert anti-urate deposition effects in the drosophila model.