菊苣根水提物对正常大鼠糖脂尿酸代谢及肝肾安全性的影响研究

Effects of Water Extract from Cichorium intybus L.Root on Serum Glucose,Lipid,and Uric Acid Metabolism in Normal Rats and Liver and Kidney Safety

目的:观察菊苣根水提物对正常大鼠糖、脂、尿酸代谢及肝肾安全性的影响。方法:SD大鼠随机分为空白对照组(水灌胃),菊苣高剂量、中剂量、低剂量组(10 mg/kg、5 mg/kg、2.5 mg/kg菊苣根水提物),别嘌醇组(20 mg/kg别嘌醇溶液),连续灌胃28 d,动态检测实验大鼠血清葡萄糖(GLU)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血清尿酸(SUA)、尿尿酸(UUA)、粪便尿酸(FUA)水平,并计算尿酸清除率(CUUA)及12 h尿尿酸排泄量;检测实验大鼠血清中尿酸代谢相关酶,如黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)水平;检测肝肾功能指标,如谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿素氮(BUN)、肌酐(Cr),并计算肝脏指数及肾脏指数。结果:实验期间,相较于空白对照组,菊苣各给药组大鼠糖、脂、尿酸代谢相关指标GLU、TC、TG、HDL-C、LDL-C、SUA、XOD、ADA、UUA、FUA水平、CUUA、12 h尿尿酸排泄量,以及肝肾安全相关指标GPT、GOT、BUN、Cr、肝脏指数及肾脏指数均无明显差异。结论:菊苣根水提物不会影响正常大鼠糖、脂及尿酸代谢,且具有较好的肝肾安全性。

Objective:To observe the effects of water extract of Cichorium intybus L.on glucose,lipid,and uric acid metabolism,as well as liver and kidney safety in normal rats.Methods:SD rats were randomly divided into a blank control group(water by gavage),high-dose,medium-dose,and low-dose C.intybus L.groups(10,5,and 2.5 mg/kg),and a allopurinol group(20 mg/kg allopurinol solution).These treatments were conducted via gavage for 28 consecutive days.Dynamic measurements of serum glucose(GLU),total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),serum uric acid(SUA),urinary uric acid(UUA),and fecal uric acid(FUA) levels were taken.Uric acid clearance rate(CUUA) and 12-hour urinary uric acid excretion were calculated.Enzyme levels in serum related to uric acid metabolism,such as xanthine oxidase(XOD) and adenosine deaminase(ADA),were also measured.Liver and kidney function indicators,including alanine aminotransferase(GPT),aspartate aminotransferase(GOT),blood urea nitrogen(BUN),and creatinine(Cr),were evaluated,and liver and kidney indices were calculated.Results:During the experimental period,there were no significant differences between the C.intybus L.groups and the blank control group in terms of glucose,lipid,and uric acid metabolism indicators(GLU,TC,TG,HDL-C,LDL-C,SUA,XOD,ADA,UUA,FUA levels,CUUA,and 12-hour urinary uric acid excretion).Additionally,there were no significant differences in liver and kidney safety indicators(GPT,GOT,BUN,Cr,liver index,and kidney index) among the groups.Conclusion:C.intybus L.root water extract does not affect glucose,lipid,or uric acid metabolism in normal rats and exhibits good liver and kidney safety.