乙肝病毒感染相关性肝病外周血Th1/Th2型细胞因子和miR-122与miR-155表达及意义

Expressions of Th1/Th2 cytokines,miR-122 and miR-155 in peripheral blood of patients with hepatitis B virus infection-associated liver disease and their significance

目的 探究乙型肝炎病毒(HBV)感染相关性肝病外周血辅助型T细胞(Th)1/Th2细胞因子和微小RNA(miR)-122与miR-155表达及意义.方法 选择2020年1月-2023年3月海安市中医院收治的HBV感染相关性肝病患者102例为研究对象,按照病情严重程度分为慢性乙型肝炎组45例、乙型肝炎肝硬化组36例、乙型肝炎肝癌组21例.另选取40名同期医院体检的健康人员为对照组.采用实时荧光聚合酶链反应检测血清中miR-122及miR-155表达水平,流式细胞仪检测Th1、Th2及Th1/Th2,电化学发光法使用全自动免疫分析仪测定肝功能指标,比较各组患者检测结果,并采用Spearman相关性分析血清Th1/Th2型细胞因子、miR-122和miR-155与病情严重程度之间的关系.结果 四组患者外周血Th1、Th2、Th1/Th2、miR-122、miR-155和肝功能指标水平比较,差异有统计学意义(P<0.05);与慢性乙型肝炎组相比,乙型肝炎肝硬化组、乙型肝炎肝癌组Th1、Th1/Th2水平降低,Th2、miR-122、miR-155水平升高,乙型肝炎肝癌组Th1、Th1/Th2水平最低,Th2、miR-122、miR-155水平最高,差异均有统计学意义(P<0.05).Spearman相关性分析结果显示,HBV感染相关性肝病血清Th1、Th1/Th2与患者病情严重程度呈负相关,Th2、miR-122和miR-155与病情严重程度呈正相关(P<0.05).结论 Th1/Th2型细胞因子、miR-122和miR-155在HBV感染相关性肝病患者血清中呈高表达状态,且与患者病情严重程度具有相关性.

OBJECTIVE To explore the expressions of helper T cell(Th)1/Th2 cytokines,microRNA(miR)-122 and miR-155 in peripheral blood of the patients with hepatitis B virus(HBV)infection-associated liver disease and analyze the significance.METHODS Totally 102 patients with HBV infection-associated liver disease who were treated in Hai'an Traditional Chinese Medicine Hospital from Jan 2020 to Mar 2023 were recruited as the research subjects and were divided into the chronic hepatitis B group with 45 cases,the hepatitis B liver cirrhosis group with 36 cases,the hepatitis B liver cancer group with 21 cases.Meanwhile,40 healthy people who received physi-cal examination were chosen as the control group.The expression levels of serum miR-122 and miR-155 were de-tected by using real-time fluorescent polymerase chain reaction;the Th1,Th2 and Th1/Th2 were detected by flow cytometry,the liver function indexes were determined by electrochemical luminescence method and automatic im-munoanalyzer.The detection results were compared among the groups.Spearman correlation analysis was per-formed for the association between the serum Th1/Th2 cytokines,miR-122 and miR-155 and the severity of ill-ness condition.RESULTS There were significant differences in the levels of peripheral blood Th1,Th2,Th1/Th2,miR-122,miR-155 and liver function indexes among the four groups(P<0.05).The levels of Th1 and Th1/Th2 of the hepatitis B liver cirrhosis group and the hepatitis B liver cancer group were significantly lower than those of the chronic hepatitis B group,while the levels of Th2,miR-122 and miR-155 of the hepatitis B liver cirrhosis group and the hepatitis B liver cancer group were significantly higher than those of the chronic hepatitis B group;the levels of Th1 and Th1/Th2 of the hepatitis B liver cancer group were the lowest,and the levels of Th2,miR-122 and miR-155 were the highest(P<0.05).The result of Spearman correlation analysis showed that the serum Th1 and Th1/Th2 of the patients with HBV infection-associated liver disease were negatively correlated with the severity of illness condition,while the Th2,miR-122 and miR-155 were positively correlated with the severity of illness condition(P<0.05).CONCLUSION The patients with HBV infection-associated liver disease show high ex-pression levels of serum Th1/Th2 cytokines,miR-122 and miR-155,which are associated with the severity of ill-ness condition.