乙丙型肝炎病毒双重感染外周血淋巴细胞亚群表达

Expressions of lymphocyte subsets in peripheral blood of patients with dual infections of hepatitis B and hepatitis C viruses

目的探讨乙丙型肝炎病毒双重感染外周血淋巴细胞亚群表达.方法选取2022年10月-2023年3月于安徽省马鞍山市传染病医院收治的67例慢性病毒性肝炎患者为研究对象.根据感染病毒类型分为慢性乙型肝炎组30例、慢性丙型肝炎组25例与双重感染组12例,另选取同期30名健康体检者为对照组.采用流式细胞技术检测各组外周血淋巴细胞亚群:总T淋巴细胞数(CD_(3)^(+))、总B淋巴细胞数(CD_(19)^(+))、辅助性T淋巴细胞数(CD_(4)^(+))、细胞毒性T淋巴细胞数(CD_(8)^(+))、NK淋巴细胞数(CD_(3)^(-)CD_(16)^(+)CD5_(6)^(+)),及CD_(4)^(+)/CD_(8)^(+)比值,分析各组之间相关性.结果不同组别的年龄与性别比较,无统计学差异;双重感染组CD_(3)^(+)、CD_(19)^(+)、CD_(4)^(+)、CD_(8)^(+)、NK细胞、CD_(4)^(+)/CD_(8)^(+)水平低于健康对照组和慢性丙型肝炎组(P<0.05),与慢性乙型肝炎组比较,无统计学差异.慢性乙型肝炎组CD_(3)^(+)、CD_(4)^(+)、NK细胞水平低于健康对照组(P<0.05);慢性乙型肝炎组CD_(3)^(+)、CD_(4)^(+)、CD_(19)^(+)、CD_(8)^(+)水平低于慢性丙型肝炎组(P<0.05);慢性丙型肝炎组NK细胞水平低于健康对照组(P<0.05).结论外周血淋巴细胞亚群检测可以作为评估乙丙型肝炎病毒双重感染、乙型肝炎及丙型肝炎的免疫水平指标,对患者的免疫治疗和预后判断具有重要意义.

OBJECTIVE To explore the expressions of lymphocyte subsets in peripheral blood of the patients with dual infections of hepatitis B virus(HBV)and hepatitis C virus(HCV).METHODS Totally 67 patients with chro-nic viral hepatitis who were treated in Maanshan Infectious Disease Hospital from Oct 2022 to Mar 2023 were re-cruited as the research subjects and were divided into the chronic hepatitis B group with 30 cases,the chronic hep-atitis C group with 25 cases and the dual infection group with 12 cases according to the type of viral infection.Meanwhile,30 healthy people who received physical examination were chosen as the control group.The lympho-cyte subsets in peripheral blood,including total counts of T lymphocytes(CD_(3)^(+)),total counts of B lymphocytes(CD_(19)^(+)),counts of helper T lymphocytes(CD_(4)^(+)),counts of cytotoxic T lymphocytes and counts of NK lympho-cytes(CD_(3)^(-)CD_(16)^(+)CD5_(6)^(+)),were detected by flow cytometry,the ratio of CD_(4)^(+)to CD_(8)^(+)was determined,and the association among the groups was observed.RESULTS There were no significant differences in the age and gender among the groups.The levels of CD_(3)^(+),CD_(19)^(+),CD_(4)^(+),CD_(8)^(+),NK cell and CD_(4)^(+)/CD_(8)^(+)of the dual infection group were significantly lower than those of the healthy control group and the chronic hepatitis C group(P<0.05),as compared with the chronic hepatitis B group,there were no significant differences.The levels of CD_(3)^(+),CD_(4)^(+)and NK cell of the chronic hepatitis B group were significantly lower than those of the healthy control group(P<0.05);the levels of CD_(3)^(+),CD_(4)^(+),CD_(19)^(+)and CD_(8)^(+)of the chronic hepatitis B group were significantly lower than those of the chronic hepatitis C virus group(P<0.05);the NK cell level of the hepatitis C group was signifi-cantly lower than that of the healthy control group(P<0.05).CONCLUSION The peripheral blood lymphocyte subsets can be used for assessment of the immune level of the patients with dual infections of HBV and HCV,hepatitis B and hepatitis C,and they have great significance for immunotherapy and assessment of prognosis.