反相高效液相色谱法同时拆分3种手性杀菌剂

Simultaneous enantiomeric separation of three triazole fungicides by reversed-phase high performance liquid chromatography

应用Chiralpak IH手性色谱柱,建立了一种同时拆分多效唑、己唑醇、糠菌唑3种三唑类杀菌剂对映异构体的高效液相色谱分析方法。采用反相色谱条件,以乙腈-水为流动相,在230 nm下进行分析,在优化条件下,即乙腈-水(40∶60, V/V)为流动相,柱温35℃、流速1.0 mL/min时,3种三唑类杀菌剂对映异构体能同时实现手性分离。

A high performance liquid chromatographic method was established for the simultaneous chiral separation of three triazole fungicides,including paclobutrazol,hexaconazole and bromuconazole,using Chiralpak IH column.The analysis of three triazole fungicides was carried out by reversed-phase high performance liquid chromatography with the mobile phase of acetonitrile and water.The wavelength of UV detector was 230 nm.Under the optimized separation conditions as follows:acetonitrile-water(40:60,V/V)as mobile phase,column temperature of 35℃and flow rate of 1.0 mL/min,three triazole fungicides were chiral separated simultaneously.