鲁米诺-还原金纳米粒子/碳纳米管三维复合材料基电化学发光传感器检测血清中的乳酸

Detection of lactate in serum by electrochemiluminescence biosensor based on luminol-reduced gold nanoparticles/carbon nanotubes three dimensional nanocomposites

采用一锅水热法制备了鲁米诺-还原金纳米粒子/碳纳米管三维复合材料(Lu-AuNPs/CNTs),用其制备固态电化学发光(ECL)传感器,并用于血清中乳酸的检测。采用场发射扫描电子显微镜(FSEM)、透射电子显微镜(TEM)、X射线粉末衍射仪(XPS)和X射线光电子能谱仪(EDS)对Lu-AuNPs/CNTs的形貌、结构、尺寸进行了表征。结果表明,碳纳米管(CNTs)为三维立体网状结构,鲁米诺-还原金纳米粒子(Lu-AuNPs)尺寸均一地分布在CNTs的表面。将Lu-AuNPs/CNTs固定在玻碳电极表面制成固态ECL传感器,其电化学发光信号约是Lu-AuNPs的2倍。该传感器的电化学发光强度与溶液中H2O2浓度成正比,线性范围为0.5~200μmol/L,检测限为0.2μmol/L。Lu-AuNPs/CNTs可用于固定乳酸氧化酶,所制备的传感器对乳酸的检测限为3.0μmol/L。该生物传感器可用于人血清样品中乳酸的测定。

A electrochemiluminescence(ECL)biosensor was constructed for the detection of lactate in serum based on a new synthesized luminol-reduced gold nanoparticles/carbon nanotubes three dimensional nanocomposites(Lu-AuNPs/CNTs)by one-pot hydrothermal method.The morphology,structure,and size of Lu-AuNPs/CNTs were characterized using field emission scanning electron microscopy(FSEM),transmission electron microscopy(TEM),X-ray powder diffractometer(XPS),and X-ray photoelectron spectroscopy(EDS).The results show that Lu-AuNPs/CNTs has a three-dimensional network structure,and the Lu-AuNPs are uniformly distributed on the surface of CNTs.A solid-state electrochemiluminescence(ECL)sensor was constructed by fixing Lu AuNPs/CNTs on the surface of a glassy carbon electrode,and the signal of Lu-AuNPs/CNTs was approximately twice of that of Lu-AuNPs.The ECL intensity of Lu-AuNPs/CNTs increased linearly with H2O2 concentration in the range from 0.5 to 200μmol/L,with a limit of detection(LOD)of 0.2μmol/L.Lactate oxidase was immobilized on Lu-AuNPs/CNTs to fabricate a lactate ECL biosensor with a LOD of 3μmol/L for lactate.This biosensor can be used for the determination of lactate in human serum.