离子液体萃取-高效液相色谱法同时测定破壁灵芝孢子粉中10种三萜化合物

Simultaneous determination of ten triterpenoids contained in the powders of broken Ganoderma lucidum spore by high-performance liquid chromatogra⁃phy combined with ionic liquids extraction

建立了离子液体萃取-高效液相色谱法测定破壁灵芝孢子粉中10种灵芝三萜化合物的分析方法。样品采用液液萃取的方法,以1-丁基-3-甲基咪唑四氟硼酸盐为分散剂,以1-己基-3-甲基咪唑六氟磷酸盐为萃取剂提取灵芝三萜类目标化合物,经Shim Nex Phenyl色谱柱分离,外标法定量。结果表明:10个三萜化合物色谱峰分离良好,在测定的质量浓度范围内线性关系良好,相关系数均大于0.999,相对标准偏差(RSD)为2.3%~9.0%,实际样品加标回收率为77.6%~91.2%。本方法适用于灵芝三萜的定量检测。

A method was proposed for the simultaneous determination of ten triterpenoids in broken Ganoderma lucidum spore powders(GLSP)by combining ionic liquid extraction with high-performance liquid chromatography(HPLC).The target Lingzhi triterpenoids were extracted through liquid-liquid extraction by taking 1-butyl-3-methylimidazolium tetrafluoroborate as dispersant and 1-hexyl-3-methylimidazolium hexafluorophosphate as extractant.Then,the extracts were separated using Shim Nex Phenyl chromatographic column and quantified by external standard method.The chromatographic peaks of ten triterpenoids were effectively separated,and the linear relationships of the ten components were satisfactory in the measured ranges of concentration.The correlation coefficients(r)exceeded 0.999,the relative standard deviations were 2.3%-9.0%,and the average recoveries were 77.6%-91.2%.This method is suitable for the determination of triterpenoids in GLSP.