厦门市种猪群猪伪狂犬病血清学调查

Serological survey of pseudorabies on breeding pigs in Xiamen city

通过对厦门市种猪群猪伪狂犬病病毒(PRV)的感染状况进行摸底调查,为指导猪场防控和净化猪伪狂犬病(PR)提供参考依据。2023年9月采集厦门市40个规模化猪场1350份种猪群猪血清样品,采用酶联免疫吸附试验(ELISA)进行PRV-gE、PRV-gB抗体检测,并对不同区域、猪群种类、不同规模及种猪群免疫次数的PR抗体进行分析。结果显示,厦门市养殖场PRV-gE抗体场阳性率为20.00%,个体阳性率为12.00%;PRV-gB抗体场合格率为87.50%,个体合格率为90.22%。与同安区相比,翔安区猪场PRV-gE抗体阳性率较高,PRV-gB抗体合格率较低。母猪的gE抗体阳性率最高(13.11%),公猪gE抗体阳性率最低(4.08%,P=0.013);后备母猪PRV-gB抗体水平最高(97.69%)。不同规模猪场比较,存栏2000头以下、2000~4000头、4000头以上养殖场PRV-gE抗体阳性率分别为3.89%、22.99%和11.07%(P<0.001),PRV-gB抗体合格率分别为90.52%、87.20%和94.30%,且均差异显著(P=0.005)。种猪免疫接种2次,其PRV-gE抗体阳性率极显著高于免疫3次(P<0.001)。厦门市种猪群猪伪狂犬病总体控制良好,部分养殖场仍存在野毒感染,还需加强免疫接种,提升管理水平,持续做好猪伪狂犬病净化工作。

Serologic infection status of pseduorabies virus(PRV)on breeding pigs in xiamen city was studied to provide references for control and decontamination of PR in pig farm.1350 sera samples of pigs from 40 pig farms were deteced the antibody of PRV-gE and PRV-gB by ELISA kits in September,2023.The PR antibody of different regions,pig species,farms of different sizes and the number of immunizations of breeding pigs were analyzed.The results showed that the farm PRV-gE antibody positive rate was 20.00%and the individual positive rate was 12.00%.The farm qualified rate of PRV-gB antibody was 87.50%,and the individual qualified rate of was 90.22%.The positive rates of PRV-gE antibody was the higher and gB immunoantibody qualified level is lower in Xiangan than Tongan.The highest gE antibody positive level was 13.11%in sows,and was lowest(4.08%)in boars(P=0.013);Reserve sows have the highest level of PRV-gB antibody(97.69%).Comparing different sizes farms,the positive rate of gE antibody in below 2000 pig stock farms,2000 to 4000 farms,and over 4000 farms were 3.89%,22.99%,11.07%(P<0.001);the qualified rates of gB antibody were 90.52%,87.20%and 94.30%(P=0.005),respectively.The farms with PR vaccine 2 times got higher PRV-gE antibody positive rates(P<0.001).The overall control of swine pseudorabies was good in the breeding herd in Xiamen,but there were still wild virus infection in some farms.It is also necessary to strengthen immunization,improve management level,and continue to do a good job in purifying porcine pseudorabies.