富硒与普通小球藻提取物成分差异及抗氧化活性比较

Comparison of Components and Antioxidant Activity between Selenium-enriched and Common Chlorella Extract

为了研究富硒小球藻提取物与普通小球藻提取物基本成分和抗氧化活性的差异及相关性,测定了富硒小球藻和普通小球藻提取物中总氮、总糖、多酚、黄酮、皂苷、总硒、多糖和多糖硒的含量,及其还原Fe^(3+)、清除DPPH·、ABTS^(+)·、羟基自由基(·OH)和超氧阴离子自由基(O_(2)^(-)·)的能力,并分析了成分与抗氧化能力之间的Person相关。结果表明,富硒小球藻提取物中总糖、多酚、黄酮、总硒、多糖和多糖硒含量显著高于普通小球藻提取物(P<0.05),但总氮和皂苷含量显著低于普通小球藻提取物(P<0.05)。富硒小球藻提取物还原Fe^(3+)、清除DPPH·和ABTS^(+)·的活性强于普通小球藻提取物,但清除·OH和O_(2)^(-)·的能力低于普通小球藻提取物。相关性分析表明含氮化合物、总糖、多酚、黄酮、总皂苷、总硒、多糖和多糖硒含量的增加均能在一定程度上增加抗氧化能力(P<0.05)。·OH清除能力与总硒和多糖硒呈显著负相关(P<0.05)。总之,富硒小球藻提取物含有较多的生理活性物质(除含氮化合物和皂苷)和较强的抗氧化活性,具有作为保健食品开发的潜力。

To investigate the difference of the components and antioxidant activity between the Se-enriched and the common chlorella extract,as well as the correlation between the components and antioxidant activity.This study determined the content of total nitrogen,total sugar,polyphenols,flavonoids,saponins,total selenium,polysaccharide and polysaccharide selenium in selenium-enriched and common chlorella extract and their ability to reduce Fe^(3+),scavenge DPPH·,ABTS^(+)·,·OH and O_(2)^(-)·,as well as the correlation between the content and antioxidant capacity.The results showed that compared with common chlorella extract,selenium-enriched chlorella extract contained more content of total sugar,polyphenol,flavonoid,total selenium,polysaccharide and polysaccharide selenium(P<0.05),but less total nitrogen and saponins(P<0.05).The Se-enriched chlorella extract had higher activity in reducing Fe^(3+)and scavenging DPPH·and ABTS^(+)·,but lower activity in scavenging·OH and O_(2)^(-)·than that of common chlorella extract.Correlation analysis showed that higher content of nitrogen,total sugar,polyphenols,flavonoids,saponins,total selenium,polysaccharide and polysaccharide selenium could strengthen the antioxidant activity(P<0.05).The·OH scavenging capacity was negatively correlated with total selenium and polysaccharide selenium(P<0.05).In general,Se-enriched chlorella extract had more physiological active substances(except total nitrogen and saponins)and stronger antioxidant activity,which would provide the potential application for Se-enriched chlorella extract in health food products.