超声辅助酶法提取金环胡蜂蜂房多糖的工艺优化及抗氧化性研究

Optimization of Ultrasonic Assisted Enzymatic Extraction of Polysaccharides from Vespa Mandarinia Nidus and its Antioxidant Activity

优化超声辅助酶法提取金环胡蜂蜂房多糖(vespa mandarinia nidus polysaccharides,VMNP)工艺条件,并分析其多糖抗氧化性,为开发利用VMNP提供参考依据。在单因素实验考察液料比、超声温度、超声时间、纤维素酶用量对VMNP提取量影响的基础上,采用响应面法优化VMNP的最佳提取条件;并以维生素C为对照,通过测定VMNP对DPPH自由基(DPPH·)和羟基自由基(·OH)的清除率,考察VMNP的抗氧化活性。结果表明:超声辅助酶法提取VMNP的最佳条件为液料比43∶1(mL/g)、超声温度46℃、超声时间47 min、纤维素酶用量4.10%,在该条件下VMNP提取量为(31.86±0.08)mg/g;所提取的VMNP对DPPH·和·OH均表现出较好的清除能力,当VMNP溶液质量浓度为0.03140 mg/mL时,对DPPH·和·OH的清除率分别达到同浓度下维生素C的80.26%和69.38%。采用超声辅助酶法能够有效提取VMNP,VMNP具有一定的抗氧化性,VMNP可作为一种抗氧化剂进行开发利用。

Optimize the process conditions for ultrasonic assisted enzymatic extraction of vespa mandarinia nidus polysaccharides(VMNP)from vespa mandarinia nidus,and analyze their antioxidant properties,providing a reference for the development and utilization of VMNP.On the basis of single factor experiments to investigate the effects of liquid-solid ratio,ultrasonic temperature,ultrasonic time,and cellulase dosage on the extraction amount of VMNP,response surface methodology was used to optimize the extraction conditions of VMNP;With vitamin C as the control,the antioxidant activity of VMNP was investigated by measuring the scavenging rate of VMNP on DPPH radical(DPPH•)and hydroxyl radical(•OH).The results showed that the optimal conditions for ultrasonic assisted enzymatic extraction of VMNP were as follows:liquid-solid ratio 43:1(mL/g),ultrasonic temperature 46℃,ultrasonic time 47 min,cellulase dosage 4.10%,under these conditions,the extraction amount of VMNP was(31.86±0.08)mg/g;When the mass concentration of VMNP solution was 0.03140 mg/mL,the clearance rates of DPPH•and•OH reached 80.26%and 69.38%of vitamin C at the same concentration,respectively.The ultrasonic assisted enzymatic method can effectively extract VMNP,which has certain antioxidant properties and can be developed and utilized as an antioxidant.