副干酪乳杆菌FP02对高尿酸血症大鼠血清尿酸水平的影响

Effect of Lactobacillus paracasei FP02 on the Serum Uric Acid Level in Hyperuricemic Rats

本研究对酸菜中分离出的一株副干酪乳杆菌FP02进行体外降尿酸性能检测、生理生化特性表征,并探究该菌株对高尿酸血症(hyperuricemia,HUA)大鼠模型血清尿酸水平的影响。体外结果显示,副干酪乳杆菌FP02能够有效降解尿酸及其前体物质肌苷、鸟苷,降解率分别为29.10%、67.09%、70.48%,且对黄嘌呤氧化酶(xanthine oxidase,XOD)活性的抑制率为64.20%。同时,该菌株可分解多种糖(醇),对胃酸、胆盐具有良好的耐受性。通过氧嗪酸钾灌胃联合高尿酸饮食构建HUA大鼠模型,连续灌胃副干酪乳杆菌FP02冻干粉14 d后,FP02治疗组的血清尿酸含量较高尿酸模型组降低了49.96%(P<0.001),接近空白对照组,且尿液中尿酸排泄量增加,血清中XOD活性受到显著抑制(P<0.001),肾切片结果显示FP02治疗组大鼠的肾损伤得到了部分恢复。该研究将为副干酪乳杆菌FP02作为预防HUA的潜在药品或功能性食品奠定了基础。

In this study,Lactobacillus paracasei FP02,a strain isolated from pickled Chinese cabbage,was tested for its capability to reduce uric acid in vitro,and its physiological and biochemical characteristics were characterized.Furthermore,the effect of this strain on the serum uric acid level of a hyperuricemic(HUA) rat model was explored.The in vitro results showed that L.paracasei FP02 degraded uric acid and its precursor substances,inosine and guanosine,by 29.10%,67.09%,and 70.48%,respectively,and inhibited xanthine oxidase(XOD) activity by 64.20%.Simultaneously,this strain could ferment various sugars(alcohols) and exhibited good tolerance to gastric acid and bile salts.The 14-day continuous intragastric administration of freeze-dried L.paracasei FP02 powder reduced the serum uric acid concentration by 49.96%compared to the hyperuricemia model group(P < 0.001),which was established by intragastric administration of potassium oxonate combined with high dietary uric acid.After the treatment,the serum uric acid concentration approached the levels observed in the blank control group.Additionally,in the FP02 treatment group,uric acid excretion in the urine increased,and serum XOD activity was significantly inhibited(P < 0.001).Renal sections showed that renal injury was partially restored in the FP02 treatment group.This study will lay the foundation for the development of L.paracasei FP02 as a potential drug or functional food for preventing HUA.