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沙门氏菌电化学适配体传感器的研究进展

Research Progress in Electrochemical Aptamer-Based Sensors for Salmonella Detection
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摘要 沙门氏菌是全球食源性疾病的重要原因,在生鲜肉类食品中检出率较高,对其进行快速、准确、灵敏检测在保障食品安全和人们身体健康方面至关重要。电化学适配体传感器由于结合了适配体的识别特异性和电化学传感器的灵敏度优势而备受关注,在实现对沙门氏菌的定性、定量检测方面具有潜力。但是,目前相关综述主要集中于传感器的制备过程,鲜有与沙门氏菌的结构和性质进行关联性分析,导致传感器的设计和改进缺乏直接的理论依据。因此,本文从沙门氏菌的结构及其性质出发,建立与适配体传感器的内在联系,从电极的修饰材料、适配体在工作电极上的固定、信号传感策略和信号放大策略4个方面进行综述,以期为沙门氏菌及其他食源性致病菌的快速检测提供参考。 Salmonella is an important cause of foodborne diseases worldwide,with a high detection rate in fresh meat.Rapid,accurate and sensitive detection of Salmonella is crucial in safeguarding food safety and people's health.Electrochemical aptamer-based sensors,integrating the advantages of both aptamers and electrochemical sensors in terms of recognition specificity and sensitivity,attract much attention,thus having the potential for application in qualitative and quantitative detection of Salmonella.However,most existing reviews focus on the preparation process of sensors,while little information is available regarding the correlation between the structure and properties of Salmonella,leading to the lack of a direct theoretical basis for the design and improvement of sensors.Therefore,based on the structure and properties of Salmonella,this article establishes its internal connection with aptamer-based sensors,and summarizes the electrode modification materials,aptamer fixation on the working electrode,the signal sensing strategies and the signal amplification strategies,in order to provide a reference for the rapid detection of Salmonella and other foodborne pathogenic bacteria.
作者 郝进华 龚梓琪 李敏 尹佳琪 何佳琦 李艳青 张春玲 张敏 HAO Jinhua;GONG Ziqi;LI Min;YIN Jiaqi;HE Jiaqi;LI Yanqing;ZHANG Chunling;ZHANG Min(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China)
出处 《食品科学》 EI CAS CSCD 北大核心 2024年第14期277-287,共11页 Food Science
基金 国家自然科学基金青年科学基金项目(31901773) 中国博士后科学基金项目(2022M712608)。
关键词 沙门氏菌 适配体 电化学传感器 固定方式 信号传感策略 Salmonella aptamer electrochemical sensors fixation mode signal sensing strategies
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