阿拉伯木聚糖、β-D-葡聚糖和α-纤维素涂层对杏果活性氧代谢的影响

Effects of Arabinoxylan,β-D-Glucan,andα-Cellulose Coating on Reactive Oxygen Species Metabolism in Apricot Fruits

使用阿拉伯木聚糖(arabinoxylan,AX)、(1,3;1,4)-β-D-葡聚糖((1,3;1,4)-β-D-glucan,BG)和α-纤维素复合溶液对采后杏果进行涂层处理,探究谷物细胞壁多糖涂层对采后杏果活性氧代谢系统的影响,并阐明其作用机理。结果表明:涂层能够有效抑制丙二醛和活性氧含量的上升,对照组的丙二醛、H2O2和O2-含量分别比ABF-C8处理组(α-纤维素质量浓度8 g/100 mL)高37.50%、33.33%和57.14%;此外,涂层处理还能维持杏果的抗氧化能力和抗氧化酶活性,其抗坏血酸和总酚含量分别比对照组高73.33%和8.40%;小麦细胞壁多糖涂层能够延缓杏果实呼吸过程中活性氧的产生和积累,从而保持细胞膜的完整性,同时涂层能够使水果和外界环境形成气体和水分之间的屏障,维持活性氧清除酶活性及抗氧化能力,从而延长采后杏果的贮藏期。

In this study,the effect of a mixed coating of the cereal cell wall polysaccharides arabinoxylan(AX),(1,3;1,4)-β-D-glucan(BG)andα-cellulose on the reactive oxygen species(ROS)metabolism system of postharvest apricot fruits and its action mechanism were investigated.The results showed that the coating could effectively inhibit the increase of–malondialdehyde(MDA)and ROS contents.The contents of MDA,H2O2,and O2 in the control group were 37.50%,33.33%,and 57.14%higher than those in the ABF-C8 treatment group(8 g/100 mLα-cellulose),respectively.In addition,the coating treatment maintained the antioxidant capacity and antioxidant enzyme activity of apricot fruits,and its ascorbic acid and total phenol contents were 73.33%and 8.40%higher than those in the control group,respectively.The polysaccharide coating could effectively retard the generation and accumulation of ROS during apricot fruit respiration,thereby preserving the integrity of the cell membrane.At the same time,the coating was able to form a barrier against gas and water between the fruit and the external environment,maintaining the activity of ROS scavenging enzymes as well as the antioxidant capacity,thus prolonging the storage period of apricot fruits after harvest.