忍冬属6种“银花”类药材在小鼠体内抗炎镇痛作用研究

Study on the anti-inflammatory and analgesic effects of six"sliver flower"medical herbs in Lonicera Linn.in mice

目的比较6种不同“银花”类药材提取物在小鼠体内抗炎镇痛活性差异,为评价其药理作用提供科学依据。方法采用二甲苯致小鼠耳肿胀的急性炎症模型和冰醋酸致小鼠扭体的致痛模型,比较细毡毛忍冬与《中华人民共和国药典》收载的5种银花类药材醇提物抗炎镇痛作用。结果6种药材醇提物均能有效降低小鼠耳肿胀度和抑制小鼠扭体(P<0.05)。在给药组中,细毡毛忍冬、灰毡毛忍冬和红腺忍冬抗炎作用与华南忍冬抗炎作用比较,差异有统计学意义(P<0.05);细毡毛忍冬、忍冬和灰毡毛忍冬能延长扭体潜伏期,忍冬和细毡毛忍冬的扭体次数较其他组显著减少,差异有统计学意义(P<0.05)。结论6种药材具有体内抗炎镇痛效果,细毡毛忍冬、灰毡毛忍冬和红腺忍冬的抗炎效果较好,细毡毛忍冬和忍冬的镇痛效果较好。

Objective To compare the differences in anti-inflammatory and analgesic activities of six different extracts of"sliver flower"medical herbs in mice,and to provide a scientific basis for evaluating their pharmacological effects.Methods An acute inflammatory model of xylene-induced ear swelling in mice and an analgesic model of glacial acetic acid-induced writhing in mice were used to compare the anti-inflammatory and analgesic effects of Lonicera similis with those of the alcoholic extracts of five genera of"sliver flower"medical herbs contained in the Pharmacopoeia of the People’s Republic of China.Results The alcoholic extracts of all six herbs were effective in reducing the swelling of mouse ears and inhibiting mouse writhing(P<0.05).In the administration group,the anti-inflammatory effects of Lonicera similis Hemsl.,Lonicera macranthoides Hand.-Mazz.,and Lonicera hypoglauca Miq.were statistically different from the anti-inflammatory effects of Lonicera confuse(Sweet)DC.,with statistically significant differences(P<0.05).Lonicera similis Hemsl.,Lonicera japonica Thunb.,and Lonicera macranthoides Hand.-Mazz.prolonged the latency period of writhing,and the frequency of writhing in mice treated with Lonicera japonica Thunb.,and Lonicera similis Hemsl.was significantly reduced compared with that in the other groups,with a statistically significant difference(P<0.05).Conclusion Six medical herbs have anti-inflammatory and analgesic effects in vivo.Lonicera similis Hemsl.,Lonicera macranthoides Hand.-Mazz.,and Lonicera hypoglauca Miq.have better anti-inflammatory effects,while Lonicera japonica Thunb.,and Lonicera similis Hemsl.have better analgesic effects.