不同pH值条件下超声波辅助水浴提取对鱼鳞明胶凝胶特性、流变特性和结构特性的影响

Effect of Ultrasonic-Assisted Water Bath Extraction at Different pH on the Gelling,Rheological and Structural Properties of Fish Scale Gelatin

对比分析不同pH值条件下的普通水浴(waterbath,WB)和超声波辅助水浴(ultrasonic-assisted waterbath,UWB)提取对草鱼鱼鳞明胶提取动力学、凝胶性质、流变特性与结构特性的影响。结果表明,当pH>7.0时,UWB作用下鱼鳞明胶的凝胶强度较WB更大。流变特性结果表明,UWB处理增强了鱼鳞明胶表观黏度,却降低了其凝胶点和融胶点。结构分析结果表明UWB可以降低鱼鳞明胶α-螺旋与β-折叠的含量,此外,pH值和UWB均可以改变鱼鳞明胶的三级结构。本研究可为实现鱼鳞废弃物的高值化综合利用提供思路。

This study comparatively analyzed the effects of water bath(WB) and ultrasonic-assisted water bath(UWB)extraction at different pH conditions on the extraction kinetics,gelling properties,rheological properties,and structural properties of grass carp fish scale gelatin.Results indicated that the gel strength of fish scale gelatin extracted by UWB was greater than that obtained by WB at pH more than 7.0.The rheological results demonstrated that UWB elevated the apparent viscosity of fish scale gelatin,but decreased the gelling and melt points.The results of structural analysis indicated that UWB resulted in a reduction in the content of α-helix and β-sheet in fish scale gelatin.Furthermore,both pH and UWB were found to alter the tertiary structure of fish scale gelatin.This study may provide novel ideas for the high-value comprehensive utilization of fish scale waste.