纸基微流控电化学芯片检测农药残留的研究进展

Research Progress on Electrochemical Paper-Based Analytical Devices for the Detection of Pesticide Residues

农药的发展推动了全球农业生产,但随之而来的残留问题对人类健康造成严重威胁,因此,亟需构建快速、准确、灵敏、经济的现场大规模检测方法。纸基微流控电化学芯片是一种高灵敏便携式的微型分析系统,它以纸为基底,依靠毛细管力驱动流体,对待测物进行分离检测。由于农药及农产品的种类繁多、基质复杂,因此,对其制备提出了更高的要求。本文首先从纸的选择、亲疏水通道的建立、分离区的集成和电极的印刷4个环节梳理了纸基微流控电化学芯片的制备过程,并基于农药的电化学检测原理,建立农药检测与芯片制备之间的联系,最后讨论了纸基微流控电化学芯片的优势、挑战和发展趋势,以期为纸基微流控电化学芯片更全面地应用于农残检测领域提供理论参考。

The development of pesticides has significantly advanced global agricultural production,but the ensuing problem of pesticide residues poses a severe threat to human health.So,there is an urgent need to establish a rapid,accurate,sensitive,cost-effective and on-site method for large-scale detection of pesticide residues.Electrochemical paper-based analytical devices(ePADs) represents a highly sensitive and portable microanalysis system,which uses paper as a substrate and relies on a capillary force to drive the fluid for the separation and detection of samples.The diversity of pesticides and agricultural products and the complexity of matrices impose high requirements on the sample preparation procedure.This article summarizes the preparation process of ePADs from the aspects of paper selection,the establishment of hydrophilic and hydrophobic channels,the integration of separation zones,and electrode printing.Subsequently,this paper establishes the relationship between pesticide detection and chip preparation based on the mechanism of electrochemical detection of pesticides.Finally,it discusses the advantages,challenges and future prospects of ePADs with a view to providing theoretical reference for enhanced application of ePADs in the field of pesticide residue detection.