白藜芦醇改善小鼠成纤维细胞机械力损伤所诱导的氧化应激

Resveratrol ameliorates the oxidative stress induced by the mechanical stress in mouse fibroblasts

目的:研究白藜芦醇(RSV)在体外机械力损伤后的抗氧化作用,并探究其是否通过线粒体相关途径促进细胞的损伤后修复。方法:以四点弯曲细胞力学加载仪对小鼠成纤维细胞L929进行机械力损伤(MS)后,经RSV进行治疗,以检测细胞的活力情况、氧化应激状态、凋亡情况和线粒体功能,在此基础上,以一种有效和选择性的Sirt1抑制剂Selisistat(Synonyms:EX-527)对RSV的治疗进行抑制,进一步探究RSV通过改善线粒体功能治疗机械力损伤所诱导的氧化应激损伤的治疗效果。结果:RSV可以有效改善机械力损伤后成纤维细胞的活力、凋亡水平和氧化应激情况,并可能通过改善线粒体功能、改善MS后细胞的功能,其机制可能与线粒体动力学相关,在受到EX-527抑制后,RSV改善机械力损伤的氧化应激的治疗效果也随之减弱。结论:RSV是一种有效的治疗小鼠成纤维细胞机械力损伤的抗氧化药物,有利于压力性尿失禁的治疗中的药物研发与应用。

Objective:To study the antioxidant effect of resveratrol(RSV)after mechanical stress‑related injury in vitro,and explore its impact on promoting cell repair of mechanical stress injury through mitochondrial‑related pathways.Methods:Mechanical stress(MS)was performed on mouse fibroblast L929 by four‑point bending cell mechanical loading apparatus before the treatment of RSV,and the cell viability,oxidative stress,apoptosis,and mitochondrial function were detected.The treatment of RSV was inhibited by a potent and selective Sirt1 inhibitor,Selisistat(Synonyms:EX‑527),to evaluate the effect of RSV on mechanical stress injury in vitro.These processes aim to evaluate and analyze the therapeutic effect of RSV on oxidative stress injury induced by mechanical force,and the result showed that it might be associated with improving mitochondrial function.Results:RSV can effectively improve fibroblasts'cell viability,apoptosis level,and oxidative stress after mechanical stress injury.It may also improve the function of cells after MS by improving mitochondrial function,and may exert therapeutic effects through mitochondrial dynamics.After being inhibited by EX‑527,the above therapeutic effects can also be weakened.Conclusion:RSV is an effective antioxidant drug for the treatment of mechanical injury of mouse fibroblasts,which is beneficial for drug development and application in the treatment of stress urinary incontinence.