鼻咽低分化鳞状细胞癌细胞系中TIGD1的表达及作用

Function of TIGD1 in poorly differentiated nasopharyngeal squamous cell carcinoma cell lines

目的:探讨触发转座衍生因子1(TIGD1)在鼻咽低分化鳞状细胞癌细胞系中的功能。方法:运用siRNA以及过表达质粒干扰CNE2和HNE1细胞系中的TIGD1基因,采用实时荧光定量PCR及Western Blot进行基因敲减以及过表达之后的效率验证;借助CCK-8的方法测量不同处理组中细胞的增殖活性;采用实时荧光定量PCR、Western Blot和流式细胞技术对不同的处理组中细胞的凋亡率、细胞的周期变化情况进行检测。结果:在CNE2和HNE1细胞系中,TIGD1基因的敲减使细胞增殖减慢,细胞的凋亡率上升,细胞周期停滞;TIGD1基因的过表达使细胞增殖加快,细胞的凋亡率下降,细胞增殖加快。结论:TIGD1在鼻咽低分化鳞状细胞癌中的高表达,可能与疾病的发生及进展相关,这可为寻找鼻咽低分化鳞状细胞癌的治疗靶点提供线索。

Objective:To investigate the function of trigger transposable element-derived 1(TIGD1)in poorly differentiated nasopharyngeal squamous cell cancer cells.Methods:siRNAs and overexpressed plasmids were used to interfere TIGD1 gene in CNE2 and HNE1 cell lines.The gene knockdown and overexpression efficiency were verified by real-time quantitative PCR and Western Blot.CCK-8 method was used to measure the proliferation activity of cells in different treatment groups.The apoptosis rate and cell cycle changes in different treatment groups were detected by real-time fluorescence quantitative PCR,Western Blot,and flow cytometry.Results:In CNE2 and HNE1 cell lines,TIGD1 knockdown slowed cell proliferation and increased cell apoptosis rate,while the cell cycle was arrested.The overexpression of the TIGD1 gene accelerated the cell proliferation,decreased the cell apoptosis rate,and accelerated the cell proliferation.Conclusion:The high expression of TIGD1 in poorly differentiated nasopharyngeal squamous cell cancer may be related to the occurrence and progression of the disease,which may provide clues for finding its therapeutic targets.