宫颈病变患者病灶组织miR-21、miR-146a、miR-224表达与HR-HPV感染、宫颈病变程度的关系

Association between the expression of miR-21,miR-146a and miR-224 in lesion tissues of patients with cervical lesions and HR-HPV infection and the degree of cervical lesions

目的分析宫颈病变患者病灶组织miR-21、miR-146a、miR-224表达与高危型人乳头状瘤病毒(HR-HPV)感染、宫颈病变程度的关系。方法选取宫颈病变患者252例,根据宫颈病变程度分为宫颈癌组、低级别鳞状上皮内病变(LSIL)组、高级别鳞状上皮内病变(HSIL)组;根据是否感染HR-HPV分为感染组和非感染组。另选宫颈正常的子宫肌瘤患者84例为对照组。PCR检测各组miR-21、miR-146a和miR-224表达水平,并分析宫颈癌患者临床病理特征与miR-21、miR-146a和miR-224表达的关系。结果感染组、非感染组miR-224水平高于对照组,感染组miR-224水平高于非感染组,宫颈癌组高于对照组、LSIL组、HSIL组(P<0.05)。低分化、FIGO分期Ⅱb~Ⅲa期、淋巴结转移、HR-HPV感染的宫颈癌患者病灶组织中miR-224高于中高分化、FIGO分期Ⅰb~Ⅱa期、无淋巴结转移及非HR-HPV感染者(P<0.05)。分化程度、HR-HPV感染、FIGO分期、淋巴结转移是影响宫颈病变患者病灶组织miR-224水平的影响因素。结论宫颈癌组织miR-224表达明显上调,且HR-HPV感染者miR-224表达水平更高;HR-HPV感染、分化程度、FIGO分期、淋巴结转移与宫颈癌组织miR-224表达密切相关。

Aim To analyze the association between the expression of miR-21,miR-146a and miR-224 in lesion tissues of patients with cervical lesions and high risk-human papilloma virus(HR-HPV)infection and the degree of cervical lesions.Methods According to the degree of cervical lesions,252 patients were divided into the cervical cancer group,the low-grade squamous intraepithelial lesion(LSIL)group and the high-grade squamous intraepithelial lesion(HSIL)group.According to the presence or absence of HR-HPV infection,the patients were divided into infection group and non-infection group.Meanwhile,84 patients with normal cervix and uterine fibroids were selected as the control group.PCR method was used to detect the expression levels of miR-21,miR-146a and miR-224 in each group.The relationship between clinicopathological characteristics of cervical cancer patients and the expression of miR-21,miR-146a and miR-224 was analyzed.Results miR-224 level in the infection group and the non-infection group was higher than that in the control group,and miR-224 level in the infection group was higher than that in the non-infection group.miR-224 level in the cervical cancer group was higher than those in the control group,LSIL group and HSIL group(P<0.05).The expression of miR-224 in lesion tissues of cervical cancer patients with low differentiation,patients at FIGO stageⅡb-Ⅲa and patients with lymph node metastasis was higher than that in patients with moderate and high differentiation,patients at FIGO stageⅠb-Ⅱa,patients without lymph node metastasis and patients without HR-HPV infection(P<0.05).Differentiation degree,HR-HPV infection,FIGO staging and lymph node metastasis were factors affecting miR-224 level in lesion tissues of patients with cervical lesions.Conclusion The expression of miR-224 is significantly up-regulated in cervical cancer tissues,and cervical cancer patients with HR-HPV infection have higher miR-224 levels.HR-HPV infection,differentiation degree,FIGO staging and lymph node metastasis are closely related to the expression of miR-224 in cervical cancer tissues.