白果仁35%乙醇-水洗脱部位多糖及多肽的含量测定

Determination of Polysaccharide and Polypeptide Content in 35%Ethanol-Water Elution Fraction of Ginkgo Kernels

目的对白果仁止咳有效部位35%乙醇-水洗脱部位中多糖及多肽的含量进行测定,为白果仁的综合开发和质量控制提供科学依据。方法采用苯酚-硫酸法、双缩脲法,建立标准曲线,并进行精密度、稳定性、重复性、加样回收率的考察。结果白果仁35%乙醇-水洗脱部位中多糖和多肽均在浓度范围内有良好的线性关系,方法学验证RSD值均小于2%,加样回收率分别为97.96%、97.94%,多糖含量为13.99%,多肽含量为31.41%。结论本研究建立的白果仁35%乙醇-水洗脱部位多糖和多肽含量测定方法简单可行,稳定性和重现性好。

Objective To determine the content of polysaccharides and polypeptides in the 35%ethanol-water elution part of the effective part of ginkgo kernels,and to provide scientific basis for the comprehensive development and quality control of ginkgo kernels.Methods The standard curve was established by the phenol-sulfuric acid method and biuret method,and the precision,stability,repeatability,and recovery rate were investigated.Results The polysaccharides and polypeptides in the 35% ethanol-water elution part of ginkgo kernel had a good linear relationship in the concentration range.The RSD values of methodological verification were less than 2%.The recovery rates were 97.96% and 97.94%.The polysaccharides content was 13.99%,and the polypeptides content was 31.41%.Conclusion The established method for the determination of polypeptides and polysaccharides in the 35% ethanol-water elution part of ginkgo kernels is simple and feasible,with good stability and reproducibility.