缺血性脑卒中SD大鼠血清、脑脊液中NSE、MBP水平及其与神经功能缺损评分和脑梗死体积的相关性

Levels of NSE and MBP in serum and CSF of SD rats with ischemic stroke and their correlation with neurological deficit score and cerebral infarct volume

目的探讨缺血性脑卒中SD大鼠血清、脑脊液中神经元特异性烯醇化酶(NSE)、髓鞘碱性蛋白(MBP)水平及其与神经功能缺损评分和脑梗死体积的相关性。方法购买SD大鼠进行大脑中动脉闭塞缺血模型造模手术,将术后成功造模的大鼠随机分为8组,每组8只,术后8 h进行神经功能缺损评分,术后在8 h、12 h、1 d、3 d、5 d、7 d及21 d时间段采集腹腔静脉血及抽取延髓脑脊液后,断头取脑,进行0.4%的2,3,5-氯化三苯基四氮唑大脑染色测定脑梗死体积,并检测NSE及MBP水平。采用Spearman相关对血清、脑脊液NSE、MBP水平与脑梗死体积和神经功能缺损评分的相关性进行分析。结果各组血清、脑脊液中NSE和MBP水平在术后8 h开始升高,3 d达峰值,7 d下降至与对照比较差异无统计意义(P>0.05);术后8 h至5 d,脑脊液NSE和MBP水平明显高于血清。血清、脑脊液NSE、MBP水平与脑梗死体积、神经功能缺损评分均呈正相关(P<0.05)。结论血清、脑脊液中NSE、MBP为脑梗死的新型标志物,通过动物实验可为临床患者标本采集推荐最佳时间窗口。

Objective To investigate the levels of neuron-specific enolase(NSE)and myelin basic protein(MBP)in serum and cerebrospinal fluid of SD rats with ischemic stroke and their correlations with neurological deficit score and cerebral infarction volume.Methods SD rats were purchased to establish the middle cerebral artery occlusion ischemia model.The successful modeling rats were randomly divided into 8 groups,8 rats in each group.The neurological deficit score was performed 8 hours after operation.At 8 h,12 h,1 d,3 d,5 d,7 d and 21 d after operation,abdominal venous blood and cerebrospinal fluid were collected,and brains were removed.0.4%2,3,5-triphenyltetrazolium chloride brain staining was used to measure cerebral infarction volume,and the levels of NSE and MBP were detected.Spearman correlation was used to analyze the correlation between serum and cerebrospinal fluid NSE,MBP levels and cerebral infarction volume and neurological deficit score.Results The levels of NSE and MBP in serum and cerebrospinal fluid began to increase at 8 h after operation,reached the peak at 3 d,and decreased to no significant difference compared with the control group at 7 d after operation in each group(P>0.05).From 8 h to 5 d after operation,the levels of NSE and MBP in CSF were significantly higher than those in serum.The levels of NSE and MBP in serum and CSF were positively correlated with cerebral infarction volume and neurological deficit score(P<0.05).Conclusion NSE and MBP in serum and cerebrospinal fluid are new markers of cerebral infarction,and the best time window for specimen collection can be recommended for clinical patients through animal experiments.