HPLC法测定雪地茶中鳞片衣酸和羊角衣酸的含量

Quantitative Determination of Squamatic Acid and Baeomycesic Acid in Thamnolia subuliformis by HPLC

目的:建立高效液相色谱法(HPLC)同时测定雪地茶中2种缩酚酸(鳞片衣酸、羊角衣酸)含量的方法。方法:采用Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-0.075%磷酸水溶液(B)为流动相,梯度洗脱(0~40 min,35%A→55%A;40~41 min,55%A→100%A;41~49 min,100%A;49~60 min,100%A→35%A),流速为1.0 mL·min^(-1),检测波长213 nm,柱温40℃,进样量10μL。结果:鳞片衣酸在0.15~0.55 mg·mL^(-1)范围内线性关系良好(R^(2)=1),方法的平均回收率为94.15%,RSD为0.21%(n=6)。羊角衣酸在0.05~0.55 mg·mL^(-1)范围内线性关系良好(R^(2)=0.9997),方法的平均回收率为97.83%,RSD为0.16%(n=6)。结论:建立的该含量测定方法结果准确,重复性和分离度好,可用于同时测定雪地茶中两种缩酚酸的含量,为雪地茶药材的质量控制提供了科学依据,对其质量标准的建立具有一定的参考意义。

Objective To establish an HPLC method for simultaneous determination of two depsides in Thamnolia subuliformis(Ehrh.)W.L.Culb..Methods With squamatic acid(SA)and baeomycesic acid(BA)as the reference compounds,HPLC method was performed on an Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm)column.Acetonitrile(A)-0.075%phosphoric acid in water(B)was used as gradient mobile phase(elution:0~40 min,35%A→55%A;40~41 min,55%A→100%A;41~49 min,100%A;49~60 min,100%A→35%A).The flow rate was 1.0 mL·min^(-1),the detected wavelength was 213 nm,the column temperature was 40℃,and the injection volume was 10μL.Results The linear range of SA was 0.15~0.55 mg·mL^(-1)(R^(2)=1),the average recovery rate of the method was 94.15%,and the RSD was 0.21%(n=6).The linear range of BA was 0.05~0.55 mg·mL^(-1)(R^(2)=0.9997),the average recovery rate of the method was 97.83%,and the RSD was 0.16%(n=6).Conclusion The established method was accurate,repeatable and had good resolution.It could be used for the simultaneous determination of SA and BA in T.subuliformis,which can be the scientific basis for better quality control of T.subuliformis.