初发干燥综合征肠道菌群特征分析

Characteristics of the gut microbiota in early-onset Sjogren's syndrome

目的 通过建立干燥综合征(Sjogren's syndrome,SS)动物模型,探究初发SS大鼠的肠道菌群变化,确定SS肠道微生物群的特征,为该疾病的早发现、早治疗提供新方向。方法 建立SPF级6~7周龄雄性Wistar大鼠初发SS模型,采集实验组(n=6)与对照组(n=6)大鼠粪便样本,进行宏基因组分析,探讨初发SS肠道微生态的变化。结果与对照组相比,初发SS组大鼠厚壁菌门(t=2.933, P=0.025)、Uroviricota(t=3.077,P=0.012)、虹吸病毒科(t=7.653,P<0.001)、消化链球菌科(t=2.243,P=0.049)、毛螺菌科(t=4.134,P=0.005)、乳杆菌属(t=6.720,P=0.001)、约氏乳杆菌(t=6.881,P=0.001)和丝状病毒(t=8.356,P<0.001)等相对丰度均显著升高;拟杆菌门(t=3.347,P=0.018)、拟杆菌科(t=7.260,P<0.001)、爱格菌科(t=3.214, P=0.009)、坦纳菌科(t=4.477, P=0.005)、阿克曼菌科(t=3.566, P=0.005)、粘液乳杆菌属(t=9.096,P<0.001)、拟杆菌属(t=4.956,P=0.002)、阿德勒菌属(t=3.221,P=0.009)、居海事城球杆菌属(t=4.546,P=0.003)、副拟杆菌属(t=4.477,P=0.005)、罗伊氏粘液乳杆菌(t=9.896,P<0.001)、阿德勒克罗伊茨菌(t=3.227,P=0.009)、单性拟杆菌(t=14.189,P<0.001)、Bacteroides_caecimuris(t=8.745,P<0.001)和Phocaeicola_vulgatus(t=4.936,P=0.002)等丰度均显著降低。结论 SS的初发阶段,肠道菌群不同水平的结构和多样性已发生改变;乳杆菌可能为SS个体潜在的肠道微生态治疗提供新的手段。

Objective To observe the changes of intestinal microflora in early-onset Sjogren's syndrome(SS)rats,and determine the characteristics of the intestinal microflora of SS,so as to provide a new direction for early detection and treatment of this disease.Methods The models of SS were established in male Wistar rats with SPF grade and 6-7 week old.The rat feces in the SS group(n=6)and control group(n=6)were collected for metagenomic analysis,and the changes in intestinal microecology of early-onset SS were observed.Results Compared with the healthy control rats,the relative abundances of Firmicutes(t=2.933,P=0.025),Uroviricota(t=3.077,P=0.012),Siphoviridae(t=7.653,P<0.001),Peptostreptococcaceae(t=2.243,P=0.049),Lachnospiraceae(t=4.134,P=0.005),Lactobacillus(t=6.720,P=0.001),Lactobacillus johnsonii(t=6.881,P=0.001)and Siphoviridae sp(t=8.356,P<0.001)significantly increased,while those of Bacteroidetes(t=3.347,P=0.018),Bacteroidaceae(t=7.260,P<0.001),Eggerthellaceae(t=3.214,P=0.009),Tannerellaceae(t=4.477,P=0.005),Akkermansiaceae(t=3.566,P=0.005),Limosilactobacillus(t=9.096,P<0.001),Bacteroides(t=4.956,P=0.002),Adlercreutzia(t=3.221,P=0.009),Phocaeicola(t=4.546,P=0.003),Parabacteroides(t=4.477,P=0.005),Limosilactobacillus reuteri(t=9.896,P<0.001),Adlercreutzia equolifaciens(t=3.227,P=0.009),Bacteroides uniformis(t=14.189,P<0.001),Bacteroides_caecimuris(t=8.745,P<0.001)and Phocaeicola vulgatus(t=4.936,P=0.002)significantly reduced.Conclusion In the initial stage of SS,the changes in the structure and diversity of gut microbiota at different levels appeared.Lactobacillus may provide a new approach for potential gut microbiota therapy in SS.