肠菌移植制备和质控实验室标准化技术规范中国专家共识(2023版)

Chinese expert consensus on standardized technical specifications for laboratory preparation and quality control of fecal microbiota transplantation(2023 edition)

肠菌移植也称粪菌移植(fecal microbiota transplantation,FMT),作为一种新兴的治疗手段已被尝试应用于多种疾病,FMT是重建肠道微生态的有效方法,是指将健康人粪便中的功能菌群处理以后移植到患者肠道内,重建新的肠道菌群,恢复肠道菌群的多样性和生物学功能,实现肠道内外疾病的治疗,达到治病的目的。在针对FMT的基础与临床研究领域,需要认识与了解FMT制备和质控的实验室检测方法。由于FMT技术的实验流程、质量管理等方面没有形成统一标准,为了明确FMT制备和质控实验室标准化技术规范,中国抗癌协会肿瘤与微生态专业委员会和中国感染免疫与微生态研究转化协作组组织有关专家共同讨论,提出《肠菌移植制备和质控实验室标准化技术规范中国专家共识(2023版)》,为FMT基础与临床领域进一步应用深入研究提供依据。

Fecal microbiota transplantation(FMT)has been attempted as an emerging therapeutic tool for a variety of diseases.FMT is an effective method to rebuild intestinal microecology,which refers to transplanting functional flora from healthy human feces into the patient's intestine after treatment to rebuild new intestinal flora and restore the diversity and biological of intestinal flora,so as to realize the treatment of diseases inside and outside the intestinal tract and achieve the purpose of curing diseases.In the field of basic and clinical research targeting FMT,there is a need to recognize and understand the laboratory assays for FMT preparation and quality control.Since there is no unified standard for the experimental procedure and quality management of FMT technology,in order to clarify the standardized laboratory technical specifications for FMT preparation and quality control,the Tumor and Microecology Professional Committee of the Chinese Anti-Cancer Association and the Chinese Infection Immunology and Microecology Research Translation Collaborative Group organized relevant experts to discuss and propose the"Chinese Expert Consensus on Standardized Technical Specifications for Laboratory Preparation and Quality Control of Fecal Microbiota Transplantation(2023 Edition)",which provides a basis for further application and in-depth research in the basic and clinical fields of FMT.