苹果MdCAXs家族分析及钙转运功能的初步分析

Preliminary analysis of MdCAXs gene family in apple fruit and identification of calcium transport function

苦痘病(BP)是一种苹果果实钙缺乏相关的生理性病害,然而关于苦痘病的发病机制尚不明确。目前发现,Ca^(2+)/H^(+)反向转运体(CAXs)可能与植物钙缺乏症的发生相关。对12个苹果MdCAXs基因家族成员的蛋白理化性质及蛋白序列进行了分析,同时对水溶性元素含量与钙转运相关基因表达量之间的相关性进行了分析,并鉴定了MdCAX2、MdCAX5和MdCAX11及去掉NRR的sMdCAX2、sMdCAX5和sMdCAX11的钙转运功能,发现MdCAX5和sMdCAX11这2个转运蛋白可能具备转运Ca2+的功能,进而参与调控苦痘病的发生与发展。

Bitter pit(BP) is a prevalent physiological disease with calcium deficiency in apple fruit,that significantly impacts its appearance quality and commercial value.However,the molecular mechanism of bitter pit remains unclear.The Ca^(2+)/H^(+)antiporter(CAXs) has been found to be associated with calcium deficiency in plants.In this study,we analyzed the physical and chemical properties of 12 Md CAXs gene family members,along with their protein sequences.We also conducted correlation analysis between water-soluble mineral element content,and calcium transport-related gene expressions.This article examined the calcium transport functions of Md CAX2,Md CAX5,and Md CAX11,as well as s Md CAX2,s Md CAX5,and s Md CAX11 with NRR removed.The study revealed that Md CAX5 and s Md CAX11 may play a role in transporting Ca2+and consequently contribute to the regulation of bitter pit disease occurrence and development.