27份新疆苹果种质资源果实多酚组分及其含量分析

Detection and analysis of polyphenol components and content in 27 Malus germplasms from Xinjiang

利用超高效液相色谱法及质谱联用技术,对来源于新疆的27份苹果种质资源果皮、果肉多酚组成及其含量进行测定,并进行聚类分析。结果表明,除石路边3外,26份苹果种质资源果皮总酚含量均高于果肉。在果皮和果肉中均检测到5类多酚,但果皮与果肉的多酚类组分数量不同,果皮为15种组分,果肉为14种组分,芦丁未在果肉中检测到。果皮多酚组分主要为表儿茶素和根皮苷,果肉多酚组分主要为表儿茶素和绿原酸。地方品种多酚含量特异性不强,野生种质资源多样性丰富。聚类分析结果表明:所有供试地方品种和4份野生种质资源聚为1类,其余野生种质资源聚为4类。筛选总酚含量最高的3024为优异种质资源。研究结果为新疆苹果种质资源遗传背景研究提供理论依据,为果实多酚利用和高多酚苹果品种培育提供基础材料。

Using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),the polyphenol components and content in the fruit peel and flesh of 27 apple germplasm resources from Xinjiang of China were determined.The differences in polyphenol components and content characteristics of apple germplasm resources in Xinjiang were studied,and cluster analysis on them was conducted.The results showed that,except Shilubian 3,the total phenolic content in the peel of all 26 apple germplasm resources was higher than that in the flesh.Five types of polyphenols were detected in both the fruit peel and flesh,but the number of polyphenol components in the peel and flesh was different,with 15 components in the peel and 14 components in the flesh.Rutin was not detected in the flesh.The polyphenol components in the peel were mainly epicatechin and phloridzin,and epicatechin and chlorogenic acid were the main polyphenol components in the flesh.The polyphenol content of landraces was not specific,and the diversity of wild resources was rich.The results of cluster analysis showed that all tested landraces and 4 wild germplasms in Xinjiang were clustered into one group,while the remaining wild germplasms were clustered into 4 groups.3024 was screened out as an excellent germplasm resource with the highest total phenolic content.The results of this study provided theoretical basis for the genetic background research of Xinjiang apple germplasm resources,and basic materials for the utilization of fruit polyphenols and the breeding of apple cultivars with high polyphenols.