TUB2基因作为葡萄白粉病菌DNA条形码的生物信息学分析

Bioinformatics analysis of TUB2 gene for DNA barcoding of the causal pathogen of powdery mildew on grape

葡萄白粉病是葡萄栽培中最重要的真菌病害之一,其病原物为专性寄生活体营养型葡萄白粉菌(Erysiphe necator)。分析了TUB2基因是否可以作为葡萄白粉菌及其近缘种菌株的DNA条形码,结果显示,TUB2基因在白粉菌属上具有明显的种间和种内遗传变异间距(Barcoding Gap),系统树分析显示TUB2基因对葡萄白粉菌及其近缘种具有有效的区分能力,因此认为TUB2基因可作为葡萄白粉病菌诊断的理想DNA条形码候选片段。

Grape powdery mildew is one of the most important fungal diseases in viticulture,and its pathogen is Erysiphe necator.In this study,we investigated whether the TUB2 gene could serve as a DNA barcoding differentiating Erysiphe necator and its closely-related species.The results revealed that the TUB2 genes show distinct discrepancies between intra-and inter-specific genetic distances in Erysiphe sepeices.The diversity of the TUB2 genes can effectively distinguish Erysiphe necator and its closely-related species via phylogenetic study.Therefore,the TUB2 region could be recommended as the ideal candidate DNA barcoding for the diagnose of grape powdery mildew.