新型H_(2)S的荧光探针制备及其在水样中的应用

Preparation of novel fluorescent probe for H_(2)S detection in water samples

以2-(3,5,5-三甲基环己-2-烯-1-基)丙二腈(TCM)和4-羟基苯甲醛为原料合成TCM-OH作为荧光团,以7-硝基-1,2,3-苯并二唑胺(NBD)作为识别单元,设计并合成了一种新型的近红外荧光探针(TCM-NBD),用于检测水样中的H_(2)S。考察了不同pH对探针响应H_(2)S的影响、探针对H_(2)S浓度和时间响应、探针的选择性和灵敏度,并推测可能的响应机理。结果显示,探针TCM-NBD对H_(2)S可以实现快速响应(<10 min);在0~25μmol/L范围内,吸光度和荧光强度与H_(2)S浓度之间均呈良好的线性关系,相关性分别为0.9947和0.9926,检测限分别为0.28、0.84μmol/L。该探针抗干扰性强,灵敏度高,可以成功应用于环境水样中的H_(2)S测定。

TCM-NBD,a novel near-infrared fluorescence probe for detection of H_(2)S in water samples,is designed and synthesized through using 4-hydroxy-phenyl-2-(3,5,5-trimethylcyclohex-2-en-1-yl)propionic nitrile,that is made from 2-(3,5,5-trimethylcyclohex-2-ene-1-yl)propionic nitrile(TCM)and 4-hydroxybenzaldehyde,as fluorescent group and using 7-nitro-1,2,3-benzodizolamine(NBD)as recognition unit.Effects of different pH values on the response of TCM-NBD probe towards H_(2)S are investigated,and the response of TCM-NBD probe to H_(2)S concentration and time,the selectivity and sensitivity of TCM-NBD probe are also explored.The possible response mechanism is speculated.Results show that this TCM-NBD probe can achieve rapid response to H_(2)S(<10 minutes);Within 0-25μmol·L^(-1) H_(2)S concentration range,there are good linear relationships between absorbance or fluorescence intensity and H_(2)S concentration,with correlations of 0.9947 and 0.9926,and detection limits of 0.28μmol·L^(-1) and 0.84μmol·L^(-1),respectively.TCM-NBD probe shows strong anti-interference and high sensitivity,and can be successfully applied to the detection of H_(2)S in environmental water samples.