口腔癌患者癌组织KLF5和KDM2A表达与临床病理特征及预后的关系

Relationship between the expression of KLF5 and KDM2A in cancer tissues of patients with oral cancer and clinicopathological characteristics and prognosis

目的探讨口腔癌患者癌组织Krüppel样因子5(KLF5)、赖氨酸去甲基化酶2A(KDM2A)表达与临床病理特征及预后的关系。方法回顾性收集2015年1月至2018年1月在自贡市第一人民医院进行手术治疗的80例口腔癌患者癌组织作为研究对象,另选取正常癌旁组织作为对照。采用免疫组织化学染色法检测口腔癌患者癌组织及癌旁组织KLF5和KDM2A表达情况,并采用Spearman相关性分析癌组织KLF5和KDM2A表达的相关性。收集口腔癌患者临床病理特征资料,分析癌组织KLF5和KDM2A表达情况与患者临床病理特征的关系,以及经多因素Cox回归分析口腔癌患者预后的影响因素,并进一步采用Kaplan-Meier法分析KLF5和KDM2A表达情况与口腔癌患者预后的关系。结果口腔癌患者癌组织KDM2A和KLF5高表达率显著高于癌旁组织KDM2A和KLF5高表达率,差异有统计学意义(P<0.05)。Spearman相关性分析结果显示,口腔癌患者癌组织KLF5和KDM2A表达呈正相关(r=0.375,P<0.05)。口腔癌患者KLF5和KDM2A表达与TNM分期、临床分期、有无淋巴结转移、有无局部浸润和分化程度有关(P<0.05)。多因素Cox回归分析结果显示,KLF5、KDM2A表达水平、TNM分期、淋巴结转移、局部浸润和分化程度均为口腔癌患者预后不良的独立危险因素(P<0.05)。癌组织KLF5、KDM2A高表达患者5年生存率[依次为38.88%(21/54)、42.37%(25/59)],均低于癌组织KLF5、KDM2A低表达患者5年生存率[依次为65.38%(17/26)、61.90%(13/21)],差异有统计学意义(χ^(2)=6.554、4.046,P<0.05)。结论口腔癌患者癌组织KLF5和KDM2A呈高表达且影响患者预后。

Objective To investigate the relationship between the expression of Krüppel-like factor 5(KLF5)and lysine demethylase 2A(KDM2A)in cancer tissues of patients with oral cancer and clinicopathological characteristics and prognosis.Methods Cancer tissues of 80 patients with oral cancer who underwent surgical treatment in the Zigong First People′s Hospital from January 2015 to January 2018 were retrospectively collected as research objects,and normal adjacent tissues were selected as controls.The expression of KLF5 and KDM2A in cancer tissues and adjacent tissues of patients with oral cancer was detected by immunohistochemical staining,and the correlation between KLF5 and KDM2A in cancer tissues was analyzed by Spearman correlation.Clinicopathological features of patients with oral cancer were collected,and the relationship between the expression of KLF5 and KDM2A in cancer tissues and clinicopathological features of patients was analyzed,as well as the prognostic factors of patients with oral cancer were analyzed by multivariate Cox regression.Kaplan-Meier method was used to analyze the relationship between the expression of KLF5 and KDM2A and the prognosis of patients with oral cancer.Results The high expression rates of KDM2A and KLF5 in oral cancer tissues were significantly higher than those in adjacent tissues,and the difference was statistically significant(P<0.05).Spearman correlation analysis showed that there was a positive correlation between KLF5 and KDM2A expression in oral cancer patients(r=0.375,P<0.05).The expressions of KLF5 and KDM2A in patients with oral cancer were correlated with TNM stage,clinical stage,lymph node metastasis,local invasion and differentiation degree(P<0.05).Multivariate Cox regression analysis showed that the expression level of KLF5 and KDM2A,TNM stage,lymph node metastasis,local invasion and differentiation degree were independent risk factors for poor prognosis in patients with oral cancer(P<0.05).The 5-year survival rates of patients with high expression of KLF5 and KDM2A in cancer tissues[38.88%(21/54),42.37%(25/59)]were lower than those of patients with low expression of KLF5 and KDM2A in cancer tissues[65.38%(17/26),61.90%(13/21)],the difference was statistically significant(χ^(2)=6.554,4.046,P<0.05).Conclusion The expression of KLF5 and KDM2A in cancer tissues of patients with oral cancer is high and affects the prognosis of patients.