实时荧光定量PCR检查在献血者乙型肝炎病毒核酸检测中的应用

Application of Real-Time Fluorescence Quantitative PCR Examination in Nucleic Acid Detection of Hepatitis B Virus in Blood Donors

目的探究献血者乙型肝炎病毒(HBV)核酸检测中,实时荧光定量PCR检查的应用价值。方法选取2022年10月至2023年9月宁德市中心血站10403份献血者标本,对其进行乙型肝炎病毒检测。ELISA检测用A、B两种不同试剂进行初检、复检,对检测阴性的血液标本进一步行核酸检测。结果经ELISA检测,A试剂初检乙型肝炎病毒表面抗原(HBsAg)阳性率为0.57%(59/10403);B试剂复检HBsAg阳性率为0.65%(68/10403)。对阴性血液标本进一步核酸检测,A试剂初检HBsAg阴性标本的阳性率为0.261%(27/10344);B试剂复检HBsAg阴性标本的阳性率为0.174%(18/10335)。结论献血者HBV检测使用核酸检测有助于提高血液筛查的准确性,保障用血安全。

Objective To explore the application value of real-time fluorescence quantitative PCR examination in Hepatitis B virus(HBV)nucleic acid detection in blood donors.Methods A total of 10403 blood donor specimens were selected from October 2022 to September 2023 from Ningde City Central Blood Station and tested for HBV.ELISA was performed with two different reagents A and B for primary and secondary testing,and further nucleic acid testing was performed on the negative blood specimens.Results The hepatitis B virus surface antigen(HbsAg)positivity rate was 0.57%(59/10403)for A reagent and 0.65%(68/10403)for B reagent.For further nucleic acid testing of negative blood specimens,the positivity rate of HBsAg-negative specimens on initial testing with reagent A was 0.261%(27/10344);the positivity rate of HBsAg-negative specimens on retesting with reagent B was 0.174%(18/10335).Conclusions The use of nucleic acid testing for HBV testing in blood donors helps to improve the accuracy of blood screening and protect blood safety.