辣椒蔗糖转运蛋白基因生物信息学及表达模式分析

Genome-wide Identification and Expression Analysis of Sucrose Uptake Transporter Genes in Pepper(Capsicum annuum L.)

蔗糖转运蛋白(sucrose uptake transporters,SUTs)调控蔗糖的运输和分配,在植物的发育和胁迫响应方面发挥作用。为探究辣椒中CaSUTs基因家族成员的功能,利用同源性搜索在辣椒基因组中鉴定到4个蔗糖转运蛋白基因。通过生物信息学手段分析研究了CaSUTs基因结构、系统发育关系和基因表达等。研究发现,CaSUTs蛋白分子量在12.47~65.56 kDa之间,理论等电点在6.00~9.32之间,均被定位在细胞膜上。根据系统发育将辣椒、番茄和拟南芥SUTs蛋白划分为4个进化分支。辣椒表达谱分析表明,CaSUTs基因在不同组织和不同处理下存在差异表达,说明CaSUTs基因具有组织特异性,在激素和胁迫处理中发挥重要作用。对辣椒材料‘XWHJ-M’的qPCR分析表明,CaSUTs基因显著受低温和盐胁迫诱导,推测CaSUTs基因家族成员可能在响应非生物胁迫中发挥作用。

Sucrose Uptake Transporters(SUT),which regulate the transportation and distribution of sucrose,play a vital role in the development and osmotic stress in plant.To explore the functions of CaSUTs family genes in pepper,four sucrose transporter genes were identified from pepper genome using the BLAST P program.The structures,phylogenetic relationships and expression patterns of CaSUTs were analyzed using the bioinformatic methods.The molecular weights of CaSUTs protein were between 12.47~65.56 kDa with the theoretical isoelectric points ranging from 6.00 and 9.32,and all of which were located on the cell membrane.According to phylogeny among pepper,tomato and Arabidopsis,SUTs proteins were divided into four evolutionary branches.Expression profile analysis in pepper showed that CaSUTs genes were expressed in different tissues and treatments,indicating that CaSUTs are tissue specific and play an important role in hormone and stress treatments.The qPCR analysis of pepper line'XWHJ-M'showed that CaSUTs were significantly induced by low temperature and salt stress,and members of CaSUTs gene family may play a role in response to abiotic stress.