碱蓬黄酮对四氯化碳诱导的雌性小鼠肝纤维化的保护作用

Protective effect of flavonoids from Suaeda glauca on carbon tetrachloride-induced liver fibrosis in female mice

目的观察碱蓬黄酮对四氯化碳诱导的小鼠肝纤维化防治作用。方法采用液相色谱-串联质谱(LC-MS/MS)分析碱蓬黄酮的组分。用四氯化碳诱导建立小鼠肝纤维化模型,通过分析小鼠血脂、肝功能、肝组织外观及病理变化,基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制剂-1(TIMP-1)及1型胶原蛋白(Col1α1)mRNA水平,阐明碱蓬黄酮对小鼠肝纤维化防治的有效性及可能的作用机制。结果碱蓬黄酮包含35种化合物。碱蓬黄酮能有效降低四氯化碳诱导的小鼠血脂水平,改善肝脏外观,减轻组织病变,恢复肝功能,下调TIMP-1和Col1α1而上调MMP-9转录水平。结论碱蓬黄酮对四氯化碳诱导的小鼠肝纤维化有较好的缓解作用。

Objective To evaluate the protective effect of flavonoids from Suaeda glauca(SGF)on mice with liver fibrosis induced by carbon tetrachloride.Methods The qualitative analysis of components in SGF was carried out by a LC-MS/MS method.The mouse liver fibrosis model was induced by carbon tetrachloride.The serum levels of lipid,liver function,appearance and pathological changes of liver,the mRNA levels of matrix metallopeptidase 9(MMP-9),tissue inhibitor of metalloproteinases 1(TIMP-1)and collagen 1α1(Col1α1)were selected to clarity the effectiveness of SGF on liver fibrosis and the possible mechanism in mice.Results Thirty-five compounds were preliminarily identified in SGF.SGF effectively lowered the serum levels of lipid,improved the liver appearance,alleviated the pathological changes of liver,restored the liver function,down-regulated the mRNA levels of TIMP-1 and Col1α1,up-regulated the mRNA level of MMP-9 in mice induced by carbon tetrachloride.Conclusion SGF alleviates carbon tetrachloride-induced liver fibrosis in mice.