miRNA-28-5p对胆管细胞癌患者预后的预测作用及对肿瘤增殖和侵袭的作用

Study of prognostic predictive role of miRNA-28-5p in cholangiocarcinoma patients and its promotion of tumor proliferation and invasion

目的:探讨miR-28-5p表达与胆管细胞癌患者预后的关系及其对肿瘤增殖和侵袭的作用。方法:纳入2017年10月—2020年12月在我院接受根治性切除的胆管细胞癌患者112例。选取其胆管细胞癌组织和相邻癌旁组织,在胆管癌细胞系TFK-1、RBE、HuH28、HuCCT-1、QBC939及正常胆管上皮细胞HIBEpic中检测miR-28-5p的表达水平。采用Kaplan-Meier曲线和Cox回归分析miR-28-5p与患者预后的关系。采用CCK-8和Transwell实验分析miR-28-5p对细胞增殖、迁移和侵袭的影响。结果:胆管细胞癌组织和细胞系中miR-28-5p表达水平均显著高于癌旁组织和正常上皮细胞。miR-28-5p低表达与患者的不良预后显著相关。下调miR-28-5p可促进胆管癌细胞的增殖、迁移和侵袭,而过表达miR-28-5p则可抑制细胞的增殖、迁移和侵袭。结论:miR-28-5p有作为胆管细胞癌患者的预后标志物和潜在治疗靶点的前景。

Objective To investigate the relationship between miR-28-5p expression and the prognosis of cholangiocarcinoma patients,as well as its functional role in tumor occurrence and development.Methods A total of 112 cholangiocarcinoma patients who underwent surgical resection were included in this study.The expression levels of miR-28-5p were detected in 112 pairs of cholangiocarcinoma tissues and adjacent non-cancerous tissues,as well as in CCA cell lines(TFK-1,RBE,HuH28,HuCCT-1,QBC939)and normal biliary epithelial cells(HIBEpic).The relationship between miR-28-5p and patient prognosis was analyzed using Kaplan-Meier curves and Cox regression analysis.The effects of miR-28-5p on cell proliferation,migration,and invasion were assessed using CCK-8 and Transwell assays.Results The expression levels of miR-28-5p in cholangiocarcinoma tissues and cell lines were significantly higher than those in adjacent non-cancerous tissues and normal epithelial cells.Additionally,low expression of miR-28-5p was significantly associated with poor prognosis in patients.Downregulation of miR-28-5p promoted proliferation,migration,and invasion of cholangiocarcinoma cells,while overexpression of miR-28-5p inhibited these processes.Conclusion miR-28-5p holds promise as a prognostic marker and potential therapeutic target for cholangiocarcinoma patients.